( We provide with Lefty2 qPCR primers for gene expression analysis, RP300126 )
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat Lefty2 Gene ORF cDNA clone expression plasmid, C-GFPSpark tag||RG80129-ACG|
|Rat Lefty2 Gene ORF cDNA clone expression plasmid, C-OFPSpark tag||RG80129-ACR|
|Rat Lefty2 Gene ORF cDNA clone expression plasmid, C-Flag tag||RG80129-CF|
|Rat Lefty2 Gene ORF cDNA clone expression plasmid, C-His tag||RG80129-CH|
|Rat Lefty2 Gene ORF cDNA clone expression plasmid, C-Myc tag||RG80129-CM|
|Rat Lefty2 Gene ORF cDNA clone expression plasmid, C-HA tag||RG80129-CY|
|Rat Lefty2 Gene ORF cDNA clone in cloning vector||RG80129-G|
|Rat Lefty2 Gene ORF cDNA clone expression plasmid, N-Flag tag||RG80129-NF|
|Rat Lefty2 Gene ORF cDNA clone expression plasmid, N-His tag||RG80129-NH|
|Rat Lefty2 Gene ORF cDNA clone expression plasmid, N-Myc tag||RG80129-NM|
|Rat Lefty2 Gene ORF cDNA clone expression plasmid, N-HA tag||RG80129-NY|
|Rat Lefty2 Gene ORF cDNA clone expression plasmid||RG80129-UT|
|Learn more about expression Vectors|
left-right determination factor 2 (Lefty2), also known as Left-right determination factor A (Lefty A), endometrial bleeding associated factor (left-right determination, factor A transforming growth factor beta superfamily), and Transforming growth factor beta-4, is a member of the TGF-beta family of proteins. This protein is secreted and plays a role in left-right asymmetry determination of organ systems during development. Lefty2/Lefty A may also play a role in endometrial bleeding. Mutations in Lefty2/Lefty A have been associated with left-right axis malformations, particularly in the heart and lungs. Some types of infertility have been associated with dysregulated expression of this gene in the endometrium. Alternative processing of this protein can yield three different products. Depletion of both Lefty1 and Lefty2 causes unchecked Nodal signaling, expansion of mesendoderm, and loss of ectoderm. The expansion of mesendoderm correlates with an extended period of rapid cellular internalization and a failure of deep-cell epiboly. The gastrulation defects of embryos depleted of Lefty1 and Lefty2 result from the deregulation of Squint signaling.