|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat Lefty2 Gene ORF cDNA clone expression plasmid, C-GFPSpark tag||RG80129-ACG|
|Rat Lefty2 Gene ORF cDNA clone expression plasmid, C-OFPSpark tag||RG80129-ACR|
|Rat Lefty2 Gene ORF cDNA clone expression plasmid, C-Flag tag||RG80129-CF|
|Rat Lefty2 Gene ORF cDNA clone expression plasmid, C-His tag||RG80129-CH|
|Rat Lefty2 Gene ORF cDNA clone expression plasmid, C-Myc tag||RG80129-CM|
|Rat Lefty2 Gene ORF cDNA clone expression plasmid, C-HA tag||RG80129-CY|
|Rat Lefty2 Gene ORF cDNA clone in cloning vector||RG80129-G|
|Rat Lefty2 Gene ORF cDNA clone expression plasmid, N-Flag tag||RG80129-NF|
|Rat Lefty2 Gene ORF cDNA clone expression plasmid, N-His tag||RG80129-NH|
|Rat Lefty2 Gene ORF cDNA clone expression plasmid, N-Myc tag||RG80129-NM|
|Rat Lefty2 Gene ORF cDNA clone expression plasmid, N-HA tag||RG80129-NY|
|Rat Lefty2 Gene ORF cDNA clone expression plasmid||RG80129-UT|
|Learn more about expression Vectors|
left-right determination factor 2 (Lefty2), also known as Left-right determination factor A (Lefty A), endometrial bleeding associated factor (left-right determination, factor A transforming growth factor beta superfamily), and Transforming growth factor beta-4, is a member of the TGF-beta family of proteins. This protein is secreted and plays a role in left-right asymmetry determination of organ systems during development. Lefty2/Lefty A may also play a role in endometrial bleeding. Mutations in Lefty2/Lefty A have been associated with left-right axis malformations, particularly in the heart and lungs. Some types of infertility have been associated with dysregulated expression of this gene in the endometrium. Alternative processing of this protein can yield three different products. Depletion of both Lefty1 and Lefty2 causes unchecked Nodal signaling, expansion of mesendoderm, and loss of ectoderm. The expansion of mesendoderm correlates with an extended period of rapid cellular internalization and a failure of deep-cell epiboly. The gastrulation defects of embryos depleted of Lefty1 and Lefty2 result from the deregulation of Squint signaling.