|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat IZUMO4 Gene ORF cDNA clone expression plasmid, C-GFPSpark tag||RG81606-ACG|
|Rat IZUMO4 Gene ORF cDNA clone expression plasmid, C-OFPSpark tag||RG81606-ACR|
|Rat IZUMO4 Gene ORF cDNA clone expression plasmid, C-Flag tag||RG81606-CF|
|Rat IZUMO4 Gene ORF cDNA clone expression plasmid, C-His tag||RG81606-CH|
|Rat IZUMO4 Gene ORF cDNA clone expression plasmid, C-Myc tag||RG81606-CM|
|Rat IZUMO4 Gene ORF cDNA clone expression plasmid, C-HA tag||RG81606-CY|
|Rat IZUMO4 Gene ORF cDNA clone in cloning vector||RG81606-G|
|Rat IZUMO4 Gene ORF cDNA clone expression plasmid, N-Flag tag||RG81606-NF|
|Rat IZUMO4 Gene ORF cDNA clone expression plasmid, N-His tag||RG81606-NH|
|Rat IZUMO4 Gene ORF cDNA clone expression plasmid, N-Myc tag||RG81606-NM|
|Rat IZUMO4 Gene ORF cDNA clone expression plasmid, N-HA tag||RG81606-NY|
|Rat IZUMO4 Gene ORF cDNA clone expression plasmid||RG81606-UT|
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Izumo is a sperm membrane protein which plays a key role in the fusion in the mouse. It has an Immunoglobulin (Ig) domain and an N-terminal domain for which neither the functions nor homologous sequences are known. Up to now, there four members has an N-terminal domain with significant homology to the N-terminal domain of Izumo. We call this domain Izumo domain. The four proteins are Izumo 1, 2, 3, and 4. Izumo domain possesses the ability to form dimers, whereas the transmembrane domain or the cytoplasmic domain or both of Izumo 1 are required for the formation of multimers of higher order. Izumo 1-3 are transmembrane proteins expressed specifically in the testis, and Izumo 4 is a soluble protein expressed in the testis and in other tissues. Izumo 1, 3, and 4 formed protein complexes on sperm, Izumo 1 forming several larger complexes and Izumo 3 and 4 forming a single larger complex. Co-immunoprecipitation studies showed the presence of other sperm proteins associated with Izumo 1, suggesting Izumo 1 forms a multiprotein membrane complex.