|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat IL25 ORF mammalian expression plasmid, C-GFPSpark tag||RG80194-ACG|
|Rat IL25 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80194-ACR|
|Rat IL25 ORF mammalian expression plasmid, C-Flag tag||RG80194-CF|
|Rat IL25 ORF mammalian expression plasmid, C-His tag||RG80194-CH|
|Rat IL25 ORF mammalian expression plasmid, C-Myc tag||RG80194-CM|
|Rat IL25 ORF mammalian expression plasmid, C-HA tag||RG80194-CY|
|Rat IL25 Gene cDNA clone plasmid||RG80194-G|
|Rat IL25 ORF mammalian expression plasmid, N-Flag tag||RG80194-NF|
|Rat IL25 ORF mammalian expression plasmid, N-His tag||RG80194-NH|
|Rat IL25 ORF mammalian expression plasmid, N-Myc tag||RG80194-NM|
|Rat IL25 ORF mammalian expression plasmid, N-HA tag||RG80194-NY|
|Rat IL25 natural ORF mammalian expression plasmid||RG80194-UT|
|Learn more about expression Vectors|
Interleukin-25 (IL-25) is a cytokine that shares sequence similarity with interleukin 17. This cytokine can induce NF-kappaB activation, and stimulate the production of interleukin 8. Both this cytokine and interleukin 17B are ligands for the cytokine receptor IL17BR. IL-25 is a member of the IL-17 family of cytokines. However, unlike the other members of this family, IL-25 promotes T helper (Th) 2 responses. IL-25 also regulates the development of autoimmune inflammation mediated by IL-17–producing T cells. IL-25 and IL-17, being members of the same cytokine family, play opposing roles in the pathogenesis of organ-specific autoimmunity. IL-25 promotes cell expansion and Th2 cytokine production when Th2 central memory cells are stimulated with thymic stromal lymphopoietin (TSLP)–activated dendritic cells (DCs), homeostatic cytokines, or T cell receptor for antigen triggering. Elevated expression of IL-25 and IL-25R transcripts was observed in asthmatic lung tissues and atopic dermatitis skin lesions, linking their possible roles with exacerbated allergic disorders. A plausible explanation that IL-25 produced by innate effector eosinophils and basophils may augment the allergic inflammation by enhancing the maintenance and functions of adaptive Th2 memory cells had been provided.