|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat IL20Ra ORF mammalian expression plasmid, C-GFPSpark tag||RG80227-ACG|
|Rat IL20Ra ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80227-ACR|
|Rat IL20Ra ORF mammalian expression plasmid, C-Flag tag||RG80227-CF|
|Rat IL20Ra ORF mammalian expression plasmid, C-His tag||RG80227-CH|
|Rat IL20Ra ORF mammalian expression plasmid, C-Myc tag||RG80227-CM|
|Rat IL20Ra ORF mammalian expression plasmid, C-HA tag||RG80227-CY|
|Rat IL20Ra Gene cDNA clone plasmid||RG80227-G|
|Rat IL20Ra ORF mammalian expression plasmid, N-Flag tag||RG80227-NF|
|Rat IL20Ra ORF mammalian expression plasmid, N-His tag||RG80227-NH|
|Rat IL20Ra ORF mammalian expression plasmid, N-Myc tag||RG80227-NM|
|Rat IL20Ra ORF mammalian expression plasmid, N-HA tag||RG80227-NY|
|Rat IL20Ra natural ORF mammalian expression plasmid||RG80227-UT|
|Learn more about expression Vectors|
Interleukin 20 receptor, alpha subunit (IL20RA/IL-20RA) also known as IL-20 receptor subunit alpha, Cytokine receptor class-II member 8, interleukin-20 receptor I, and interleukin-20 receptor subunit alpha, is a subunit for the interleukin-20 receptor. IL20RA/IL-20RA belongs to the type II cytokine receptor family. This cytokine is a receptor for interleukin 20 (IL20), a cytokine that may be involved in epidermal function. The receptor of IL20 is a heterodimeric receptor complex consisting of this protein and interleukin 20 receptor beta (IL20B). IL20RA forms heterodimer with IL20RB, and the complex serves as a receptor for IL19, IL20 and IL24. All three are capable of signaling through IL-20RA/IL-20RB complex. The ligand binding to receptor B creating a high-affinity binding site for the receptor A which is recruited to complete the complex. In addition, IL20RA also forms a heterodimer with the unique and specific receptor IL10RB and functions as the receptor for IL26. IL20RA is widely expressed with highest levels in skin, testis and brain. The expression of both IL20RA and IL20RB is found to be upregulated in psoriatic skin lesions on keratinocytes.