|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat IL18R1 ORF mammalian expression plasmid, C-GFPSpark tag||RG80191-ACG|
|Rat IL18R1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80191-ACR|
|Rat IL18R1 ORF mammalian expression plasmid, C-Flag tag||RG80191-CF|
|Rat IL18R1 ORF mammalian expression plasmid, C-His tag||RG80191-CH|
|Rat IL18R1 ORF mammalian expression plasmid, C-Myc tag||RG80191-CM|
|Rat IL18R1 ORF mammalian expression plasmid, C-HA tag||RG80191-CY|
|Rat IL18R1 Gene cDNA clone plasmid||RG80191-G|
|Rat IL18R1 ORF mammalian expression plasmid, N-Flag tag||RG80191-NF|
|Rat IL18R1 ORF mammalian expression plasmid, N-His tag||RG80191-NH|
|Rat IL18R1 ORF mammalian expression plasmid, N-Myc tag||RG80191-NM|
|Rat IL18R1 ORF mammalian expression plasmid, N-HA tag||RG80191-NY|
|Rat IL18R1 natural ORF mammalian expression plasmid||RG80191-UT|
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Interleukin-18 receptor 1 (IL18R1) also known as CD218 antigen-like family member A, CDw218a, IL1 receptor-related protein and CD218a, is an interleukin receptor of the immunoglobulin superfamily. IL18R1 is found expressed in lung, leukocytes, spleen, liver, thymus, prostate, small intestine, colon, placenta, and heart, and is absent from brain, skeletal muscle, pancreas, and kidney. High level of expression is found in Hodgkin disease cell lines. This receptor is specifically binds interleukin 18 (IL18), and is essential for IL18 mediated signal transduction. IL18R1 contains 3 Ig-like C2-type (immunoglobulin-like) domains and 1 TIR domain. It is a single-pass type I membrane protein. IFN-alpha and IL12 are reported to induce the expression of this receptor in NK and T cells. The increased expression of IL18R1 may contribute pathogenically to disease and is therefore a potential therapeutic target. The absence of a genetic association in the IL18R1 gene itself suggests regulation from other parts of the genome, or as part of the inflammatory cascade in multiple sclerosis without a prime genetic cause.