( We provide with IFNA4 qPCR primers for gene expression analysis, RP300166 )
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat IFNA4 Gene ORF cDNA clone expression plasmid, C-GFPSpark tag||RG80173-ACG|
|Rat IFNA4 Gene ORF cDNA clone expression plasmid, C-OFPSpark tag||RG80173-ACR|
|Rat IFNA4 Gene ORF cDNA clone expression plasmid, C-Flag tag||RG80173-CF|
|Rat IFNA4 Gene ORF cDNA clone expression plasmid, C-His tag||RG80173-CH|
|Rat IFNA4 Gene ORF cDNA clone expression plasmid, C-Myc tag||RG80173-CM|
|Rat IFNA4 Gene ORF cDNA clone expression plasmid, C-HA tag||RG80173-CY|
|Rat IFNA4 Gene ORF cDNA clone in cloning vector||RG80173-G|
|Rat IFNA4 Gene ORF cDNA clone expression plasmid, N-Flag tag||RG80173-NF|
|Rat IFNA4 Gene ORF cDNA clone expression plasmid, N-His tag||RG80173-NH|
|Rat IFNA4 Gene ORF cDNA clone expression plasmid, N-Myc tag||RG80173-NM|
|Rat IFNA4 Gene ORF cDNA clone expression plasmid, N-HA tag||RG80173-NY|
|Rat IFNA4 Gene ORF cDNA clone expression plasmid||RG80173-UT|
|Learn more about expression Vectors|
Interferon, alpha 4 (IFNA4) belongs to the alpha/beta interferon family. Two variants of IFNA4 (IFNA4a and IFNA4b) are known, which differ from each other by changes in their coding regions at nucleotide positions 220 and 410 and can be distinguished by selective restriction enzyme analysis. Interferons are produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase. IFN-alpha, the first cytokine to be produced by recombinant DNA technology, has emerged as an important regulator of growth and differentiation, affecting cellular communication and signal transduction pathways as well as immunological control. Originally discovered as an antiviral substance, the efficacy of IFN-alpha in malignant, viral, immunological, angiogenic, inflammatory, and fibrotic diseases suggests a spectrum of interrelated pathophysiologies. IFN-alpha emerged as a prototypic tumor suppressor protein that represses the clinical tumorigenic phenotype in some malignancies capable of differentiation.