|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat GFRA2 ORF mammalian expression plasmid, C-GFPSpark tag||RG80136-ACG|
|Rat GFRA2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80136-ACR|
|Rat GFRA2 ORF mammalian expression plasmid, C-Flag tag||RG80136-CF|
|Rat GFRA2 ORF mammalian expression plasmid, C-His tag||RG80136-CH|
|Rat GFRA2 ORF mammalian expression plasmid, C-Myc tag||RG80136-CM|
|Rat GFRA2 ORF mammalian expression plasmid, C-HA tag||RG80136-CY|
|Rat GFRA2 Gene cDNA clone plasmid||RG80136-G|
|Rat GFRA2 ORF mammalian expression plasmid, N-Flag tag||RG80136-NF|
|Rat GFRA2 ORF mammalian expression plasmid, N-His tag||RG80136-NH|
|Rat GFRA2 ORF mammalian expression plasmid, N-Myc tag||RG80136-NM|
|Rat GFRA2 ORF mammalian expression plasmid, N-HA tag||RG80136-NY|
|Rat GFRA2 natural ORF mammalian expression plasmid||RG80136-UT|
|Learn more about expression Vectors|
GFRA2 is a member of the GDNF receptor family. It is a glycosylphosphatidylinositol(GPI)-linked cell surface receptor for both GDNF and NTN, and mediates activation of the RET tyrosine kinase receptor. GFRA2 is a potent survival factor for central and peripheral neurons, and is essential for the development of kidneys and the enteric nervous system. Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) are its binding ligand which are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. GDNF promotes the formation of a physical complex between GFRA/GDNFRa and the orphan tyrosin kinase receptor Ret, thereby inducing its tyrosine phosphorylation. The RET is a receptor tyrosine kinase representing the signal-transducing molecule of a multisubunit surface receptor complex for the GDNF, in which GFRA/GDNFRa acts as the ligand-binding component. Experiments have improved that GFRA2 genetic variants and age may play a role in Tardive dyskinesia (TD) susceptibility, but further work is required to confirm these findings.