|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat GFRA2 Gene ORF cDNA clone expression plasmid, C-GFPSpark tag||RG80136-ACG|
|Rat GFRA2 Gene ORF cDNA clone expression plasmid, C-OFPSpark tag||RG80136-ACR|
|Rat GFRA2 Gene ORF cDNA clone expression plasmid, C-Flag tag||RG80136-CF|
|Rat GFRA2 Gene ORF cDNA clone expression plasmid, C-His tag||RG80136-CH|
|Rat GFRA2 Gene ORF cDNA clone expression plasmid, C-Myc tag||RG80136-CM|
|Rat GFRA2 Gene ORF cDNA clone expression plasmid, C-HA tag||RG80136-CY|
|Rat GFRA2 Gene ORF cDNA clone in cloning vector||RG80136-G|
|Rat GFRA2 Gene ORF cDNA clone expression plasmid, N-Flag tag||RG80136-NF|
|Rat GFRA2 Gene ORF cDNA clone expression plasmid, N-His tag||RG80136-NH|
|Rat GFRA2 Gene ORF cDNA clone expression plasmid, N-Myc tag||RG80136-NM|
|Rat GFRA2 Gene ORF cDNA clone expression plasmid, N-HA tag||RG80136-NY|
|Rat GFRA2 Gene ORF cDNA clone expression plasmid||RG80136-UT|
|Learn more about expression Vectors|
GFRA2 is a member of the GDNF receptor family. It is a glycosylphosphatidylinositol(GPI)-linked cell surface receptor for both GDNF and NTN, and mediates activation of the RET tyrosine kinase receptor. GFRA2 is a potent survival factor for central and peripheral neurons, and is essential for the development of kidneys and the enteric nervous system. Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) are its binding ligand which are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. GDNF promotes the formation of a physical complex between GFRA/GDNFRa and the orphan tyrosin kinase receptor Ret, thereby inducing its tyrosine phosphorylation. The RET is a receptor tyrosine kinase representing the signal-transducing molecule of a multisubunit surface receptor complex for the GDNF, in which GFRA/GDNFRa acts as the ligand-binding component. Experiments have improved that GFRA2 genetic variants and age may play a role in Tardive dyskinesia (TD) susceptibility, but further work is required to confirm these findings.