|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat GDNF Gene ORF cDNA clone expression plasmid, C-GFPSpark tag||RG80050-ACG|
|Rat GDNF Gene ORF cDNA clone expression plasmid, C-OFPSpark tag||RG80050-ACR|
|Rat GDNF Gene ORF cDNA clone expression plasmid, C-Flag tag||RG80050-CF|
|Rat GDNF Gene ORF cDNA clone expression plasmid, C-His tag||RG80050-CH|
|Rat GDNF Gene ORF cDNA clone expression plasmid, C-Myc tag||RG80050-CM|
|Rat GDNF Gene ORF cDNA clone expression plasmid, C-HA tag||RG80050-CY|
|Rat GDNF Gene ORF cDNA clone in cloning vector||RG80050-M|
|Rat GDNF Gene ORF cDNA clone expression plasmid, N-Flag tag||RG80050-NF|
|Rat GDNF Gene ORF cDNA clone expression plasmid, N-His tag||RG80050-NH|
|Rat GDNF Gene ORF cDNA clone expression plasmid, N-Myc tag||RG80050-NM|
|Rat GDNF Gene ORF cDNA clone expression plasmid, N-HA tag||RG80050-NY|
|Rat GDNF Gene ORF cDNA clone expression plasmid||RG80050-UT|
|Learn more about expression Vectors|
Glial cell line-derived neurotrophic factor(GDNF) is an important member of the GDNF family of ligands(GFL). The GDNF family of ligands is comprised by four neurotrophic factors: glial cell line-derived neurotrophic factor (GDNF), neurturin (NRTN), artemin (ARTN), and persephin (PSPN). It has been found that GFLs play a role in a number of biological processes including cell survival, neurite outgrowth, cell differentiation and cell migration. As the founding member, GDNF plays a key role in the promotion of the survival of dopaminergic neurons. GDNF is a highly conserved neurotrophic factor. The recombinant form of this protein also promotes the survival and differentiation of dopaminergic neurons in culture, and was able to prevent apoptosis of motor neurons induced by axotomy. GDNF also regulates kidney development and spermatogenesis, and it affects alcohol consumption. It has been shown that GDNF results in two Parkinson's disease clinical trial and in a number of animal trials. It has been taken as a potent survival factor for central motoneurons.