|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat EPCAM ORF mammalian expression plasmid, C-GFPSpark tag||RG80306-ACG|
|Rat EPCAM ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80306-ACR|
|Rat EPCAM ORF mammalian expression plasmid, C-Flag tag||RG80306-CF|
|Rat EPCAM ORF mammalian expression plasmid, C-His tag||RG80306-CH|
|Rat EPCAM ORF mammalian expression plasmid, C-Myc tag||RG80306-CM|
|Rat EPCAM ORF mammalian expression plasmid, C-HA tag||RG80306-CY|
|Rat EPCAM Gene cDNA clone plasmid||RG80306-G|
|Rat EPCAM ORF mammalian expression plasmid, N-Flag tag||RG80306-NF|
|Rat EPCAM ORF mammalian expression plasmid, N-His tag||RG80306-NH|
|Rat EPCAM ORF mammalian expression plasmid, N-Myc tag||RG80306-NM|
|Rat EPCAM ORF mammalian expression plasmid, N-HA tag||RG80306-NY|
|Rat EPCAM natural ORF mammalian expression plasmid||RG80306-UT|
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Epithelial Cell Adhesion Molecule (EpCAM), also known as GA733-2 antigen, is a type â… transmembrane glycoprotein composed of an extracellular domain with two EGF-Like repeats and a cystenin-rich region, a transmembrane domain and a cytoplasmic domain. It modulates cell adhesion and proliferation. Its overexpression has been detected in many epithelial tumours and has been associated with high stage, high grade and a worse survival in some tumour types. EpCAM has been shown to function as a calcium-independent homophilic cell adhesion molecule that does not exhibit any obvious relationship to the four known cell adhesion molecule superfamilies. However, recent insights have revealed that EpCAM participates in not only cell adhesion, but also in proliferation, migration and differentiation of cells. In addition, recent study revealed that EpCAM is the Wnt-beta-catenin signaling target gene and may be used to facilitate prognosis. It has oncogenic potential and is activated by release of its intracellular domain, which can signal into the cell nucleus by engagement of elements of the wnt pathway.