|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat EFNB3 ORF mammalian expression plasmid, C-GFPSpark tag||RG80112-ACG|
|Rat EFNB3 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80112-ACR|
|Rat EFNB3 ORF mammalian expression plasmid, C-Flag tag||RG80112-CF|
|Rat EFNB3 ORF mammalian expression plasmid, C-His tag||RG80112-CH|
|Rat EFNB3 ORF mammalian expression plasmid, C-Myc tag||RG80112-CM|
|Rat EFNB3 ORF mammalian expression plasmid, C-HA tag||RG80112-CY|
|Rat EFNB3 Gene cDNA clone plasmid||RG80112-G|
|Rat EFNB3 ORF mammalian expression plasmid, N-Flag tag||RG80112-NF|
|Rat EFNB3 ORF mammalian expression plasmid, N-His tag||RG80112-NH|
|Rat EFNB3 ORF mammalian expression plasmid, N-Myc tag||RG80112-NM|
|Rat EFNB3 ORF mammalian expression plasmid, N-HA tag||RG80112-NY|
|Rat EFNB3 natural ORF mammalian expression plasmid||RG80112-UT|
|Learn more about expression Vectors|
Ephrin B3 belongs to the ephrin family. Ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. Ephrin B3 is important in brain development as well as in its maintenance. It is especially important for forebrain function since its expression levels were particularly high in several forebrain subregions compared to other brain subregions. Ephrin B3 binds to, and induce the collapse of, commissural axons/growth cones in vitro. It may play a role in constraining the orientation of longitudinally projecting axons.