|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat EDAR ORF mammalian expression plasmid, C-GFPSpark tag||RG80228-ACG|
|Rat EDAR ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80228-ACR|
|Rat EDAR ORF mammalian expression plasmid, C-Flag tag||RG80228-CF|
|Rat EDAR ORF mammalian expression plasmid, C-His tag||RG80228-CH|
|Rat EDAR ORF mammalian expression plasmid, C-Myc tag||RG80228-CM|
|Rat EDAR ORF mammalian expression plasmid, C-HA tag||RG80228-CY|
|Rat EDAR Gene cDNA clone plasmid||RG80228-G|
|Rat EDAR ORF mammalian expression plasmid, N-Flag tag||RG80228-NF|
|Rat EDAR ORF mammalian expression plasmid, N-His tag||RG80228-NH|
|Rat EDAR ORF mammalian expression plasmid, N-Myc tag||RG80228-NM|
|Rat EDAR ORF mammalian expression plasmid, N-HA tag||RG80228-NY|
|Rat EDAR natural ORF mammalian expression plasmid||RG80228-UT|
|Learn more about expression Vectors|
Tumor necrosis factor receptor superfamily member EDAR is a Single-pass type I membrane protein. Edar was expressed reiteratively in signaling centers regulating key steps in morphogenesis. activin signaling from mesenchyme induces the expression of the TNF receptor edar in the epithelial signaling centers, thus making them responsive to Wnt-induced ectodysplasin from the nearby ectoderm. This is the first demonstration of integration of the Wnt, activin, and TNF signaling pathways. Defects in EDAR are a cause of ectodermal dysplasia anhidrotic (EDA), also known ectodermal dysplasia hypohidrotic autosomal recessive (HED). Ectodermal dysplasia defines a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. EDA is characterized by sparse hair (atrichosis or hypotrichosis), abnormal or missing teeth and the inability to sweat due to the absence of sweat glands.