Contactin-1 cDNA ORF Clone, Rat, N-DDK (Flag®) tag

Cat: RG80315-NF
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Contactin-1 cDNA ORF Clone, Rat, N-DDK (Flag®) tag General Information
Gene
Species
Rat
NCBI Ref Seq
RefSeq ORF Size
3066 bp
Description
Full length Clone DNA of Rat contactin 1 with N terminal Flag tag.
Plasmid
Promoter
Enhanced CMV promoter
Vector
pCMV3-SP-N-FLAG
Tag Sequence
FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
Sequencing Primers
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
Quality Control
The plasmid is confirmed by full-length sequencing.
Screening
Antibiotic in E.coli
Kanamycin
Antibiotic in Mammalian cell
Hygromycin
Application
Stable or Transient mammalian expression
Storage & Shipping
Shipping
Each tube contains lyophilized plasmid.
Storage
The lyophilized plasmid can be stored at ambient temperature for three months.

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

Contactin-1 Background Information

Contactins are a subgroup of molecules belonging to the immunoglobulin superfamily that are expressed exclusively in the nervous system. The subgroup consists of six members: Contactin-1, Contactin-2 (TAG-1), Contactin-3 (BIG-1), BIG-2, Contactin-5 (NB-2) and NB-3. Since their identification in the late 1980s, Contactin-1 and Contactin-2 have been studied extensively. Axonal expression and the neurite extension activity of Contactin-1 and Contactin-2 attracted researchers to study the function of these molecules in axon guidance during development. Contactin-1 and Contactin-2 have come to be known as the principal molecules in the function and maintenance of myelinated neurons. In contrast, the function of the other four members of this subgroup remained unknown until recently. Contactin-1 is a cell surface adhesion molecule that is normally expressed by neurons and oligodendrocytes. Particularly high levels of Contactin-1 are present during brain development. Contactin-1 and Contactin-2 are differentially expressed in a number of neuronal tissues during development, and they interact with several ligands including Nr-CAM, L1, NCAM, neurocan, phosphacan, and tenascin. As a cell adhesion molecule, Contactin-1 plays a role in the formation of axon connections in the developing nervous system. It was demonstrated that Contactin-1 participates in signal pathways via its association with Contactin-associated protein (CNTNAP1), receptor protein tyrosine phosphatase beta (RPTPb) and NOTCH1. Contactin-1 is also involved in paranodal axo-glial junction formation and oligodendrocytes generation. Furthermore, studies indicated that Contactin-1 functions importantly in the invasion and metastasis of lung adenocarcinoma cells. Contactin-1 may also significantly influence the functional expression and distribution of Na+ channels in neurons.

Full Name
contactin 1
References
  • Kazarinova NK, et al. (2001) Contactin associates with Na+ channels and increases their functional expression. J Neurosci. 21 (19):7517-25.
  • Eckerich C, et al. (2006) Contactin is expressed in human astrocytic gliomas and mediates repulsive effects. Glia. 53(1):1-12.
  • Su JL, et al. (2006) Knockdown of contactin-1 expression suppresses invasion and metastasis of lung adenocarcinoma. Cancer research 66 (5):2553-61.
  • Compton AG, et al. (2008) Mutations in contactin-1, a neural adhesion and neuromuscular junction protein, cause a familial form of lethal congenital myopathy. Am J Hum Genet. 83 (6):714-24.
  • Mikami T, et al. (2009) Contactin-1 is a functional receptor for neuroregulatory chondroitin sulfate-E. J Biol Chem. 284(7):4494-9.
  • Contactin-1 cDNA ORF Clone, Rat, Related Products

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    Contactin-1 cDNA ORF Clone, Rat, C-HA tag RG80315-CY
    Contactin-1 cDNA ORF Clone, Rat, N-HA tag RG80315-NY
    Contactin-1 cDNA ORF Clone, Rat, C-Myc tag RG80315-CM
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    Contactin-1 cDNA ORF Clone, Mouse, Related Products

    Product Name Catalog#(PDF)
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    Contactin-1 cDNA ORF Clone, Human, Related Products

    Product Name Catalog#(PDF)
    Contactin-1 cDNA ORF Clone in Cloning Vector, Human HG10383-M
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    Contactin-1 cDNA ORF Clone, Human, C-OFPSpark® tag HG10383-ACR
    Contactin-1 cDNA ORF Clone, Human, C-DDK (Flag®) tag HG10383-CF
    Contactin-1 cDNA ORF Clone, Human, N-DDK (Flag®) tag HG10383-NF
    Contactin-1 cDNA ORF Clone, Human, C-His tag HG10383-CH
    Contactin-1 cDNA ORF Clone, Human, N-His tag HG10383-NH
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    Contactin-1 cDNA ORF Clone, Human, N-HA tag HG10383-NY
    Contactin-1 cDNA ORF Clone, Human, C-Myc tag HG10383-CM
    Contactin-1 cDNA ORF Clone, Human, N-Myc tag HG10383-NM
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