|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat CNTF ORF mammalian expression plasmid, C-GFPSpark tag||RG80012-ACG|
|Rat CNTF ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80012-ACR|
|Rat CNTF ORF mammalian expression plasmid, N-GFPSpark tag||RG80012-ANG|
|Rat CNTF ORF mammalian expression plasmid, N-OFPSpark / RFP tag||RG80012-ANR|
|Rat CNTF ORF mammalian expression plasmid, C-Flag tag||RG80012-CF|
|Rat CNTF ORF mammalian expression plasmid, C-His tag||RG80012-CH|
|Rat CNTF ORF mammalian expression plasmid, C-Myc tag||RG80012-CM|
|Rat CNTF ORF mammalian expression plasmid, C-HA tag||RG80012-CY|
|Rat CNTF Gene cDNA clone plasmid||RG80012-M|
|Rat CNTF ORF mammalian expression plasmid, N-Flag tag||RG80012-NF|
|Rat CNTF ORF mammalian expression plasmid, N-His tag||RG80012-NH|
|Rat CNTF ORF mammalian expression plasmid, N-Myc tag||RG80012-NM|
|Rat CNTF ORF mammalian expression plasmid, N-HA tag||RG80012-NY|
|Rat CNTF natural ORF mammalian expression plasmid||RG80012-UT|
|Learn more about expression Vectors|
Ciliary neurotrophic factor (CNTF) is a member of the cytokine family. It is a polypeptide hormone that have functions in promoting neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. It's actions appear to be restricted to the nervous system. Ciliary neurotrophic factor (CNTF) has biological effects through the activation of a multi- subunit receptor complex, consisting of an extracelluar CNTF binding subunit (CNTFα) and two transmembrane signal transduction proteins: glycoprotein gp130 and LIF receptor. CNTF is considered as a potent survival factor of neurons and oligodendrocyteands may be relevant in reducing tissue destruction during inflammatory attacks. CNTF also is a survival factor for neurons of the peripheral sensory sympathetic and ciliary ganglia. It has been reported that CNTF could be an agent that has therapeutic potential and possibly induces differentiation of large multipolar ganglionic phenotype in a subset of progenitors.