( We provide with CD83 qPCR primers for gene expression analysis, RP300303 )
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat CD83 Gene ORF cDNA clone expression plasmid, C-GFPSpark tag||RG80333-ACG|
|Rat CD83 Gene ORF cDNA clone expression plasmid, C-OFPSpark tag||RG80333-ACR|
|Rat CD83 Gene ORF cDNA clone expression plasmid, C-Flag tag||RG80333-CF|
|Rat CD83 Gene ORF cDNA clone expression plasmid, C-His tag||RG80333-CH|
|Rat CD83 Gene ORF cDNA clone expression plasmid, C-Myc tag||RG80333-CM|
|Rat CD83 Gene ORF cDNA clone expression plasmid, C-HA tag||RG80333-CY|
|Rat CD83 Gene ORF cDNA clone in cloning vector||RG80333-G|
|Rat CD83 Gene ORF cDNA clone expression plasmid, N-Flag tag||RG80333-NF|
|Rat CD83 Gene ORF cDNA clone expression plasmid, N-His tag||RG80333-NH|
|Rat CD83 Gene ORF cDNA clone expression plasmid, N-Myc tag||RG80333-NM|
|Rat CD83 Gene ORF cDNA clone expression plasmid, N-HA tag||RG80333-NY|
|Rat CD83 Gene ORF cDNA clone expression plasmid||RG80333-UT|
|Learn more about expression Vectors|
The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD83 is considered as a marker of mature dendritic cells as well as an adhesion receptor that binds to resting monocytes and a subset of activated CD8+ T cells. In certain conditions, CD83 tended to dimerize or even multimerize through its aberrant intermolecular disulfide bonds. The injection of CD83-Ig can significantly enhaunce the rate of tumor growth and inhibit the T cell growth.