|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat CD55 ORF mammalian expression plasmid, C-GFPSpark tag||RG80317-ACG|
|Rat CD55 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80317-ACR|
|Rat CD55 ORF mammalian expression plasmid, C-Flag tag||RG80317-CF|
|Rat CD55 ORF mammalian expression plasmid, C-His tag||RG80317-CH|
|Rat CD55 ORF mammalian expression plasmid, C-Myc tag||RG80317-CM|
|Rat CD55 ORF mammalian expression plasmid, C-HA tag||RG80317-CY|
|Rat CD55 Gene cDNA clone plasmid||RG80317-G|
|Rat CD55 ORF mammalian expression plasmid, N-Flag tag||RG80317-NF|
|Rat CD55 ORF mammalian expression plasmid, N-His tag||RG80317-NH|
|Rat CD55 ORF mammalian expression plasmid, N-Myc tag||RG80317-NM|
|Rat CD55 ORF mammalian expression plasmid, N-HA tag||RG80317-NY|
|Rat CD55 natural ORF mammalian expression plasmid||RG80317-UT|
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CD55, also well known as decay-accelerating factor (DAF), is a member of the RCA (regulators of complement activation) family characterized by four to 30 SCRs (short consensus repeats) in their plasma-exposed regions. It is a major regulator of the alternative and classical pathways of complement activation and is expressed on all serum-exposed cells. CD55 is physiologically acting as an inhibitor of the complement system, but is also broadly expressed in malignant tumours. DAF seems to exert different functions beyond its immunological role such as promotion of tumorigenesis, decrease of complement mediated tumor cell lysis, autocrine loops for cell rescue and evasion of apoptosis, neoangiogenesis, invasiveness, cell motility. It is commonly hijacked by invading pathogens, including many enteroviruses and uropathogenic Escherichia coli, to promote cellular attachment prior to infection. This 70-75 kDa glycoprotein CD55 containing four SCR modules is involved in the regulation of the complement cascade. It inhibits complement activation by suppressing the function of C3/C5 convertases, thereby limiting local generation or deposition of C3a/C5a and membrane attack complex (MAC or C5b-9) production. DAF has been identified as a ligand for an activation-associated, seven-transmembrane lymphocyte receptor, CD97, which is a receptor mediating attachment and infection of several viruses and bacteria. In addition, it has been shown that DAF regulates the interplay between complement and T cell immunity in vivo, and thus may be implicated in immune and tumor biology.