|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat CD5 ORF mammalian expression plasmid, C-GFPSpark tag||RG80374-ACG|
|Rat CD5 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80374-ACR|
|Rat CD5 ORF mammalian expression plasmid, C-Flag tag||RG80374-CF|
|Rat CD5 ORF mammalian expression plasmid, C-His tag||RG80374-CH|
|Rat CD5 ORF mammalian expression plasmid, C-Myc tag||RG80374-CM|
|Rat CD5 ORF mammalian expression plasmid, C-HA tag||RG80374-CY|
|Rat CD5 Gene cDNA clone plasmid||RG80374-G|
|Rat CD5 ORF mammalian expression plasmid, N-Flag tag||RG80374-NF|
|Rat CD5 ORF mammalian expression plasmid, N-His tag||RG80374-NH|
|Rat CD5 ORF mammalian expression plasmid, N-Myc tag||RG80374-NM|
|Rat CD5 ORF mammalian expression plasmid, N-HA tag||RG80374-NY|
|Rat CD5 natural ORF mammalian expression plasmid||RG80374-UT|
|Learn more about expression Vectors|
The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD5 is a member of the CD system. CD5 was found to be widely distributed in T-cells and B1 cells which is a subset of IgM-secreting B cells. CD5 also was found expressed in small lymphocytic lymphoma, hairy cell leukaemia and mantle cell lymphoma cells. CD5 serves to weaken the activating stimulus from the BCR so that the B1 cells can only reflect to the very strong stimuli but not the normal tissue proteins.