|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rat CD3d/CD3 delta Gene ORF cDNA clone expression plasmid, C-GFPSpark tag||RG80313-ACG|
|Rat CD3d/CD3 delta Gene ORF cDNA clone expression plasmid, C-OFPSpark tag||RG80313-ACR|
|Rat CD3d/CD3 delta Gene ORF cDNA clone expression plasmid, C-Flag tag||RG80313-CF|
|Rat CD3d/CD3 delta Gene ORF cDNA clone expression plasmid, C-His tag||RG80313-CH|
|Rat CD3d/CD3 delta Gene ORF cDNA clone expression plasmid, C-Myc tag||RG80313-CM|
|Rat CD3d/CD3 delta Gene ORF cDNA clone expression plasmid, C-HA tag||RG80313-CY|
|Rat CD3d/CD3 delta Gene ORF cDNA clone in cloning vector||RG80313-G|
|Rat CD3d/CD3 delta Gene ORF cDNA clone expression plasmid, N-Flag tag||RG80313-NF|
|Rat CD3d/CD3 delta Gene ORF cDNA clone expression plasmid, N-His tag||RG80313-NH|
|Rat CD3d/CD3 delta Gene ORF cDNA clone expression plasmid, N-Myc tag||RG80313-NM|
|Rat CD3d/CD3 delta Gene ORF cDNA clone expression plasmid, N-HA tag||RG80313-NY|
|Rat CD3d/CD3 delta Gene ORF cDNA clone expression plasmid||RG80313-UT|
|Learn more about expression Vectors|
T-cell surface glycoprotein CD3 delta chain, also known as CD3D, is a single-pass type I membrane protein. CD3D, together with CD3-gamma, CD3-epsilon and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. The majority of T cell receptor (TCR) complexes in mice and humans consist of a heterodimer of polymorphic TCRalpha and beta chains along with invariant CD3gamma, delta, epsilon, and zeta chains. CD3 chains are present as CD3gammaepsilon, deltaepsilon, and zetazeta dimers in the receptor complex and play critical roles in the antigen receptor assembly, transport to the cell surface, and the receptor-mediated signal transduction. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. This complex is critical for T-cell development and function, and represents one of the most complex transmembrane receptors. The T cell receptor-CD3 complex is unique in having ten cytoplasmic immunoreceptor tyrosine-based activation motifs (ITAMs). CD3D contains 1 ITAM domain and has been shown to interact with CD8A. In the mouse, knockout of CD3delta allows some degree of T lymphocyte differentiation since mature CD4 and CD8 as well as TCRgammadelta T lymphocytes are observed in the periphery. In contrast, deleterious mutation of the CD3delta encoding gene in the human leads to a severe combined immunodeficiency characterised by the complete absence of mature T cell subpopulations including TCRalpha/beta and TCRgamma/delta. Defects in CD3D cause severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T-/B+/NK+ SCID) which is a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. In humans the absence of CD3 delta results in a complete arrest in thymocyte development at the stage of double negative to double positive transition and the development of gamma delta T-cell receptor-positive T cells is also impaired.