( We provide with CD2 qPCR primers for gene expression analysis, RP300214 )
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Rat CD2 Gene ORF cDNA clone expression plasmid, C-GFPSpark tag||RG80158-ACG|
|Rat CD2 Gene ORF cDNA clone expression plasmid, C-OFPSpark tag||RG80158-ACR|
|Rat CD2 Gene ORF cDNA clone expression plasmid, C-Flag tag||RG80158-CF|
|Rat CD2 Gene ORF cDNA clone expression plasmid, C-His tag||RG80158-CH|
|Rat CD2 Gene ORF cDNA clone expression plasmid, C-Myc tag||RG80158-CM|
|Rat CD2 Gene ORF cDNA clone expression plasmid, C-HA tag||RG80158-CY|
|Rat CD2 Gene ORF cDNA clone in cloning vector||RG80158-G|
|Rat CD2 Gene ORF cDNA clone expression plasmid, N-Flag tag||RG80158-NF|
|Rat CD2 Gene ORF cDNA clone expression plasmid, N-His tag||RG80158-NH|
|Rat CD2 Gene ORF cDNA clone expression plasmid, N-Myc tag||RG80158-NM|
|Rat CD2 Gene ORF cDNA clone expression plasmid, N-HA tag||RG80158-NY|
|Rat CD2 Gene ORF cDNA clone expression plasmid||RG80158-UT|
|Learn more about expression Vectors|
T-cell surface antigen CD2, also known as T-cell surface antigen T11/Leu-5, and SRBC, is a single-pass type I membrane protein. It contains one Ig-like C2-type domain and one Ig-like V-type domain. CD2 is a cell adhesion molecule expressed on T cells and is recognized as a target for CD48 (rats) and CD58 (humans). CD2 has been shown to set quantitative thresholds in T cell activation both in vivo and in vitro. Further, intracellular CD2 signaling pathways and networks are being discovered by the identification of several cytosolic tail binding proteins. CD2 interacts with lymphocyte function-associated antigen (LFA-3) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain of CD2 is implicated in the signaling function. The complex of CD2 and CD58 also plays an important role in enhancing the adhesion of T lymphocytes to target cells, and promoting hyperplasia and activation of T lymphocytes. As a cell surface glycoprotein, CD2 expressed on most human T cells and natural killer (NK) cells and plays an important role in mediating cell adhesion in both T-lymphocytes and in signal transduction.