C1s qPCR Primer Pairs, Rat

Cat: RP301154
Contact us:
+86-400-890-9989
order@sinobiological.com
Distributors
All C1s Reagents
CRO Services
Product recommended

All C1s Reagents
C1s qPCR Primer Pairs, Rat General Information
Target Details
Species:
Rat
Product Details
Oligo-Type:
qPCR Primers
Component:
1 vial of lyophilized qPCR primer mix (1 nmol each primer, sufficient for 200 numbers of 25 μl reactions).
QPCR Primer Description:
Verified forward and reverse primers for analyzing the quantitative expression of gene.
Application & Quality
Application:
SYBR® Green-based quantitative real-time PCR (qPCR).
Quality Control:
The primer mix has been verified to generate satisfactory qPCR data on Roche Applied-science LightCycler® 480 Ⅱ.
Storage & Shipping
Shipping:
Lyophilized qPCR primer mix is shipped at ambiente temperatura
Storage:
The lyophilized product is stable for one year from date of receipt when stored at -20℃. The suspended product is stable for six months from date of receipt when stored at -20℃.

***Sino biological qEASY qPCR primer pairs are used for SYBR Green-based real-time RT-PCR, The primers are designed by using SBI's proprietary primer design algorithm. Our primer collection covers the entire human genomes. It can be widely applied in the quantitative analysis of gene expression.***

Features and Advantages
Unique Primer Design
To avoid genomic DNA amplification, at least one primer is designed crosses the junction of exons according to the conserved region of a specific gene with all variants.
Strict Validation Process
Confirmed in positive organizations; screened the primer with high specificity and high sensitivity.
Uniform PCR conditions, Saving time and cost
~100% amplification curve, ensuring the accuracy of the RNA quantitative
C1s Background Information

Complement is an integral component of the adaptive and innate immune systems and represents one of the major effector systems for the immune responses. The classical complement pathway is triggered by C1, a complex composed of the binding protein C1q and two proenzymes, C1r and C1s. Upon binding of IgG to the head of C1q, C1r undergoes autoactivation and in turn cleaves and activates C1s. C1r and C1s, the proteases responsible for activation and proteolytic activity of the C1 complex of complement, share similar overall structural organizations featuring five nonenzymic protein modules (two CUB modules surrounding a single EGF module, and a pair of CCP modules) followed by a serine protease domain. Besides highly specific proteolytic activities, both proteases exhibit interaction properties associated with their N-terminal regions. In contrast, C1r and C1s widely differ from each other by their glycosylation patterns: both proteins contain Asn-linked carbohydrates, but four glycosylation sites are present on C1r, and only two on C1s. As a highly specific serine protease, C1s executes the catalytic function of the C1 complex: the cleavage of C4 and C2, and thus instigates a sequence of activation steps of other components of the complement system, culminating in the formation of the membrane attack complex which induces cell lysis. Like other complement serine proteases C1s has restricted substrate specificity and it is engaged into specific interactions with other subcomponents of the complement system. The only other protein known to interact with C1s physiologically is SerpinC1, an inhibitor of serine protease, which inhibits C1s activity and thus plays a regulatory role in controlling the function of C1s enzyme.

Full Name
complement component 1, s subcomponent
References
  • Arlaud GJ, et al. (1989) Structure and function of C1r and C1s: current concepts. Behring Inst Mitt. (84): 56-64.
  • Thielens NM, et al. (1999) Structure and functions of the interaction domains of C1r and C1s: keystones of the architecture of the C1 complex. Immunopharmacology. 42(1-3): 3-13.
  • Gl P, et al. (2002) C1s, the protease messenger of C1. Structure, function and physiological significance. Immunobiology. 205(4-5): 383-94.
  • C1s qPCR Primer Pairs, Rat, Related Products

    Product Name Catalog#(PDF)
    C1s cDNA ORF Clone in Cloning Vector, Rat RG81198-U
    C1s cDNA ORF Clone, Rat, untagged RG81198-UT
    C1s Lentiviral cDNA ORF Clone, Rat, C-GFPSpark® tag RG81198-ACGLN
    C1s cDNA ORF Clone, Rat, C-GFPSpark® tag RG81198-ACG
    C1s cDNA ORF Clone, Rat, C-OFPSpark® tag RG81198-ACR
    C1s cDNA ORF Clone, Rat, C-DDK (Flag®) tag RG81198-CF
    C1s cDNA ORF Clone, Rat, N-DDK (Flag®) tag RG81198-NF
    C1s cDNA ORF Clone, Rat, C-His tag RG81198-CH
    C1s cDNA ORF Clone, Rat, N-His tag RG81198-NH
    C1s cDNA ORF Clone, Rat, C-HA tag RG81198-CY
    C1s cDNA ORF Clone, Rat, N-HA tag RG81198-NY
    C1s cDNA ORF Clone, Rat, C-Myc tag RG81198-CM
    C1s cDNA ORF Clone, Rat, N-Myc tag RG81198-NM

    C1s qPCR Primer Pairs, Mouse, Related Products

    Product Name Catalog#(PDF)
    C1s cDNA ORF Clone in Cloning Vector, Mouse MG50979-G
    C1s cDNA ORF Clone, Mouse, untagged MG50979-UT
    C1s Lentiviral cDNA ORF Clone, Mouse, C-GFPSpark® tag MG50979-ACGLN
    C1s cDNA ORF Clone, Mouse, C-GFPSpark® tag MG50979-ACG
    C1s cDNA ORF Clone, Mouse, C-OFPSpark® tag MG50979-ACR
    C1s cDNA ORF Clone, Mouse, C-DDK (Flag®) tag MG50979-CF
    C1s cDNA ORF Clone, Mouse, N-DDK (Flag®) tag MG50979-NF
    C1s cDNA ORF Clone, Mouse, C-His tag MG50979-CH
    C1s cDNA ORF Clone, Mouse, N-His tag MG50979-NH
    C1s cDNA ORF Clone, Mouse, C-HA tag MG50979-CY
    C1s cDNA ORF Clone, Mouse, N-HA tag MG50979-NY
    C1s cDNA ORF Clone, Mouse, C-Myc tag MG50979-CM
    C1s cDNA ORF Clone, Mouse, N-Myc tag MG50979-NM

    C1s qPCR Primer Pairs, Human, Related Products

    Product Name Catalog#(PDF)
    C1s cDNA ORF Clone in Cloning Vector, Human HG10220-G
    C1s cDNA ORF Clone, Human, untagged HG10220-UT
    C1s Lentiviral cDNA ORF Clone, Human, C-GFPSpark® tag HG10220-ACGLN
    C1s cDNA ORF Clone, Human, C-GFPSpark® tag HG10220-ACG
    C1s cDNA ORF Clone, Human, C-OFPSpark® tag HG10220-ACR
    C1s cDNA ORF Clone, Human, C-DDK (Flag®) tag HG10220-CF
    C1s cDNA ORF Clone, Human, C-DDK (Flag®) tag HG10220-G-F
    C1s cDNA ORF Clone, Human, N-DDK (Flag®) tag HG10220-NF
    C1s cDNA ORF Clone, Human, C-His tag HG10220-CH
    C1s cDNA ORF Clone, Human, N-His tag HG10220-NH
    C1s cDNA ORF Clone, Human, C-HA tag HG10220-CY
    C1s cDNA ORF Clone, Human, N-HA tag HG10220-NY
    C1s cDNA ORF Clone, Human, C-Myc tag HG10220-CM
    C1s cDNA ORF Clone, Human, N-Myc tag HG10220-NM
    info info
    添加购物车成功! 添加购物车失败!