APE1 qPCR Primer Pairs, Rat

Cat: RP300720
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APE1 qPCR Primer Pairs, Rat General Information
Target Details
Species:
Rat
Product Details
Oligo-Type:
qPCR Primers
Component:
1 vial of lyophilized qPCR primer mix (1 nmol each primer, sufficient for 200 numbers of 25 μl reactions).
QPCR Primer Description:
Verified forward and reverse primers for analyzing the quantitative expression of gene.
Application & Quality
Application:
SYBR® Green-based quantitative real-time PCR (qPCR).
Quality Control:
The primer mix has been verified to generate satisfactory qPCR data on Roche Applied-science LightCycler® 480 Ⅱ.
Storage & Shipping
Shipping:
Lyophilized qPCR primer mix is shipped at ambiente temperatura
Storage:
The lyophilized product is stable for one year from date of receipt when stored at -20℃. The suspended product is stable for six months from date of receipt when stored at -20℃.

***Sino biological qEASY qPCR primer pairs are used for SYBR Green-based real-time RT-PCR, The primers are designed by using SBI's proprietary primer design algorithm. Our primer collection covers the entire human genomes. It can be widely applied in the quantitative analysis of gene expression.***

Features and Advantages
Unique Primer Design
To avoid genomic DNA amplification, at least one primer is designed crosses the junction of exons according to the conserved region of a specific gene with all variants.
Strict Validation Process
Confirmed in positive organizations; screened the primer with high specificity and high sensitivity.
Uniform PCR conditions, Saving time and cost
~100% amplification curve, ensuring the accuracy of the RNA quantitative
APE1 Background Information

The enzyme is known to be a redox factor (Ref-1) stimulating DNA binding activity of AP-1 binding proteins such as Fos and Jun as well as a multifunctional DNA repair enzyme having 5' AP endonuclease, DNA 3' repair diesterase, 3'-5' exonuclease and DNA 3'-phosphatase activities.Although Apex mRNA was expressed ubiquitously, the levels varied significantly, suggesting organ- or tissue-specific expression of the Apex gene. The highest level was observed in the testis, relatively high levels in the thymus, spleen, kidney and brain, and the lowest level in the liver in rats. However, the present results suggested that APEX/Ref-1 gene product can interact with AP-1 binding proteins in brain, especially in the hippocampal formation, to regulate some brain functions by redox-activation.

Full Name
APEX nuclease (multifunctional DNA repair enzyme) 1
References
  • Ono Y, et al. (1995) Developmental expression of APEX nuclease, a multifunctional DNA repair enzyme, in mouse brains. Brain Res Dev Brain Res.86 (1-2): 1-6.
  • Tan Y, et al. (1996) cDNA cloning of rat major AP endonuclease (APEX nuclease) and analyses of its mRNA expression in rat tissues. Acta Med Okayama. 50 (1): 53-60.
  • Yao M, et al. (1999) Genomic structure of the rat major AP endonuclease gene (Apex) with an adjacent putative O-sialoglycoprotease gene (Prsmg1/Gcpl1) and a processed Apex pseudogene (Apexp1). Acta Med Okayama. 53 (6): 245-52.
  • APE1 qPCR Primer Pairs, Rhesus, Related Products

    Product Name Catalog#(PDF)
    APE1 cDNA ORF Clone in Cloning Vector, Rhesus CG90434-G
    APE1 cDNA ORF Clone, Rhesus, untagged CG90434-UT
    APE1 Lentiviral cDNA ORF Clone, Rhesus, C-GFPSpark® tag CG90434-ACGLN
    APE1 cDNA ORF Clone, Rhesus, C-GFPSpark® tag CG90434-ACG
    APE1 cDNA ORF Clone, Rhesus, N-GFPSpark® tag CG90434-ANG
    APE1 cDNA ORF Clone, Rhesus, C-OFPSpark® tag CG90434-ACR
    APE1 cDNA ORF Clone, Rhesus, N-OFPSpark® tag CG90434-ANR
    APE1 cDNA ORF Clone, Rhesus, C-DDK (Flag®) tag CG90434-CF
    APE1 cDNA ORF Clone, Rhesus, N-DDK (Flag®) tag CG90434-NF
    APE1 cDNA ORF Clone, Rhesus, C-His tag CG90434-CH
    APE1 cDNA ORF Clone, Rhesus, N-His tag CG90434-NH
    APE1 cDNA ORF Clone, Rhesus, C-HA tag CG90434-CY
    APE1 cDNA ORF Clone, Rhesus, N-HA tag CG90434-NY
    APE1 cDNA ORF Clone, Rhesus, C-Myc tag CG90434-CM
    APE1 cDNA ORF Clone, Rhesus, N-Myc tag CG90434-NM

    APE1 qPCR Primer Pairs, Rat, Related Products

    Product Name Catalog#(PDF)
    APE1 cDNA ORF Clone in Cloning Vector, Rat RG80756-U
    APE1 cDNA ORF Clone, Rat, untagged RG80756-UT
    APE1 Lentiviral cDNA ORF Clone, Rat, C-GFPSpark® tag RG80756-ACGLN
    APE1 cDNA ORF Clone, Rat, C-GFPSpark® tag RG80756-ACG
    APE1 cDNA ORF Clone, Rat, N-GFPSpark® tag RG80756-ANG
    APE1 cDNA ORF Clone, Rat, C-OFPSpark® tag RG80756-ACR
    APE1 cDNA ORF Clone, Rat, N-OFPSpark® tag RG80756-ANR
    APE1 cDNA ORF Clone, Rat, C-DDK (Flag®) tag RG80756-CF
    APE1 cDNA ORF Clone, Rat, N-DDK (Flag®) tag RG80756-NF
    APE1 cDNA ORF Clone, Rat, C-His tag RG80756-CH
    APE1 cDNA ORF Clone, Rat, N-His tag RG80756-NH
    APE1 cDNA ORF Clone, Rat, C-HA tag RG80756-CY
    APE1 cDNA ORF Clone, Rat, N-HA tag RG80756-NY
    APE1 cDNA ORF Clone, Rat, C-Myc tag RG80756-CM
    APE1 cDNA ORF Clone, Rat, N-Myc tag RG80756-NM

    APE1 qPCR Primer Pairs, Mouse, Related Products

    Product Name Catalog#(PDF)
    APE1 cDNA ORF Clone in Cloning Vector, Mouse MG51694-U
    APE1 cDNA ORF Clone, Mouse, untagged MG51694-UT
    APE1 Lentiviral cDNA ORF Clone, Mouse, C-GFPSpark® tag MG51694-ACGLN
    APE1 cDNA ORF Clone, Mouse, C-GFPSpark® tag MG51694-ACG
    APE1 cDNA ORF Clone, Mouse, N-GFPSpark® tag MG51694-ANG
    APE1 cDNA ORF Clone, Mouse, C-OFPSpark® tag MG51694-ACR
    APE1 cDNA ORF Clone, Mouse, N-OFPSpark® tag MG51694-ANR
    APE1 cDNA ORF Clone, Mouse, C-DDK (Flag®) tag MG51694-CF
    APE1 cDNA ORF Clone, Mouse, N-DDK (Flag®) tag MG51694-NF
    APE1 cDNA ORF Clone, Mouse, C-His tag MG51694-CH
    APE1 cDNA ORF Clone, Mouse, N-His tag MG51694-NH
    APE1 cDNA ORF Clone, Mouse, C-HA tag MG51694-CY
    APE1 cDNA ORF Clone, Mouse, N-HA tag MG51694-NY
    APE1 cDNA ORF Clone, Mouse, C-Myc tag MG51694-CM
    APE1 cDNA ORF Clone, Mouse, N-Myc tag MG51694-NM

    APE1 qPCR Primer Pairs, Human, Related Products

    Product Name Catalog#(PDF)
    APE1 cDNA ORF Clone in Cloning Vector, Human HG16161-G
    APE1 cDNA ORF Clone, Human, untagged HG16161-UT
    APE1 Lentiviral cDNA ORF Clone, Human, C-GFPSpark® tag HG16161-ACGLN
    APE1 cDNA ORF Clone, Human, C-GFPSpark® tag HG16161-ACG
    APE1 cDNA ORF Clone, Human, N-GFPSpark® tag HG16161-ANG
    APE1 cDNA ORF Clone, Human, C-OFPSpark® tag HG16161-ACR
    APE1 cDNA ORF Clone, Human, N-OFPSpark® tag HG16161-ANR
    APE1 cDNA ORF Clone, Human, C-DDK (Flag®) tag HG16161-CF
    APE1 cDNA ORF Clone, Human, N-DDK (Flag®) tag HG16161-NF
    APE1 cDNA ORF Clone, Human, C-His tag HG16161-CH
    APE1 cDNA ORF Clone, Human, N-His tag HG16161-NH
    APE1 cDNA ORF Clone, Human, C-HA tag HG16161-CY
    APE1 cDNA ORF Clone, Human, N-HA tag HG16161-NY
    APE1 cDNA ORF Clone, Human, C-Myc tag HG16161-CM
    APE1 cDNA ORF Clone, Human, N-Myc tag HG16161-NM
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