Rabbit Monoclonal Antibody

Rabbit Monoclonal Antibody Products (CLICK to see product Info. and price)

Advantages of Rabbit Monoclonal Antibody (RabMAb)

Monoclonal antibodies are traditionally made by fusing myeloma cells with the spleen cells from a mouse that has been immunized with the desired antigen. Using a mouse as a host is cost-effective and easy to manage. However, researchers have seen limitations of mouse monoclonal antibodies. First, Many protein targets are highly conserved between mice and humans, and can therefore be recognized as self-antigens by a mouse host, making them less immunogenic. Second, mouse monoclonal antibodies have low affinities, and thus have a weak attraction to bind to the protein targets. These problems can be avoided when using rabbit as a host animal. The availability of rabbit monoclonal antibodies is highly desirable for their high specificity and affinity, and greatly improved response to mouse antigens. In addition, rabbits are known to produce antibodies to many antigens that are not especially immunogenic in mice. Rabbit monoclonal antibodies also have other advantages, such as more diverse epitope recognition and improved immune response to small-size epitopes, compared with mouse monoclonal antibodies. However, rabbit monoclonal antibodies are much more difficult to develop compared to mouse or rat monoclonal antibodies, due to lack of a stable fusion partner cell line for hybridoma development.

Our Rabbit Monoclonal Antibody Features

  • Full-length protein immunization, recognize multiple epitopes
  • Leading 2nd generation rabbit Mab technology
  • High affinity, low detection limit (as low as 1 pg/well in ELISA assay)
  • Validated in multiple applications: WB, ELISA, IHC, IF, FCM, etc.


Rabbit Monoclonal Antibodies used in IHC, IF, FCM

anti-mFCGR1 rabbit mAb anti-CD147 rabbit mAb

FCGR1 was detected in fixed paraffin embedded sections of mouse brain, using rabbit mAb 50086-R001   CD147 was detected in fixed paraffin embedded sections of human esophagus, using rabbit mAb 10186-R125

anti-CD147 rabbit mAb anti-BACE1 rabbit mAb

Immunofluorescent analysis of human CD147 in HeLa cells, using rabbit mAb 10186-R125   Flow cytometric analysis of human BACE1 expression in Jurkat cells, using rabbit mAb 10064-R027-F

Additional Antibody Products

Features of Our Rabbit Monoclonal Antibody (RabMAb) Development Platform

Sino Biological has developed a proprietary platform for efficient development of high affinity and high specificity rabbit monoclonal antibodies. We are the first company that is able to generate 2nd generation high-affinity rabbit Mab owning to our creative proprietary technology. By using recombinant protein based antigen for rabbit immunization, we can obtain rabbit monoclonal antibodies for ELISA assay as well as Western blotting, IHC, and flow cytometry applications. Sino Biological has successfully developed ELISA assays with rabbit monoclonal antibodies with pg assay sensitivity. Our rabbit monoclonal antibodies usually have binding affinity in the range of 1-100 picomole. Our rabbit monoclonal antibody platform is one of the kind in the industry and enables us to offer the most comprehensive one-stop custom antibody development services including mouse and rabbit monoclonal antibodies.

Rabbit Monoclonal Antibody (RabMAb) Related Studies

    1. H Spieker-Polet, et al. (1995) Rabbit monoclonal antibodies: generating a fusion partner to produce rabbit-rabbit hybridomas.Proc Natl Acad Sci 92(20): 9348–9352.
    2. Rief N, et al. (1998) Production and characterization of a rabbit monoclonal antibody against human CDC25C phosphatase. Hybridoma. 17(4):389-94.
    3. Cheuk W, et al. (2004) Consistent immunostaining for cyclin D1 can be achieved on a routine basis using a newly available rabbit monoclonal antibody. Am J Surg Pathol. 28(6):801-7.
    4. Huang Z, et al. (2005) Development of new rabbit monoclonal antibody to estrogen receptor: immunohistochemical assessment on formalin-fixed, paraffin-embedded tissue sections. Appl Immunohistochem Mol Morphol. 13(1):91-5.
    5. Ricardo SA, et al. (2006) HER2 evaluation using the novel rabbit monoclonal antibody SP3 and CISH in tissue microarrays of invasive breast carcinomas. J Clin Pathol. 60(9):1001-5.
    6. Wludarski SC, et al. (2008) High concordance of SP3 rabbit monoclonal antibody with FISH to evaluate HER2 in breast carcinoma. Appl Immunohistochem Mol Morphol. 16(5):466-70.
    7. van der Vegt B, et al. (2009) Validation of the 4B5 rabbit monoclonal antibody in determining Her2/neu status in breast cancer. Mod Pathol. 22(7):879-86.