Rabbit Monoclonal Antibody
Monoclonal antibodies are traditionally made by fusing myeloma cells with the spleen cells from a mouse that has been immunized with the desired antigen. Using a mouse as a host is cost-effective and easy to manage. However, researchers have seen limitations of mouse monoclonal antibodies. First, Many protein targets are highly conserved between mice and humans, and can therefore be recognized as self-antigens by a mouse host, making them less immunogenic. Second, mouse monoclonal antibodies have low affinities, and thus have a weak attraction to bind to the protein targets. These problems can be avoided when using rabbit as a host animal. The availability of rabbit monoclonal antibodies is highly desirable for their high specificity and affinity, and greatly improved response to mouse antigens. In addition, rabbits are known to produce antibodies to many antigens that are not especially immunogenic in mice. Rabbit monoclonal antibodies also have other advantages, such as more diverse epitope recognition and improved immune response to small-size epitopes, compared with mouse monoclonal antibodies. However, rabbit monoclonal antibodies are much more difficult to develop compared to mouse or rat monoclonal antibodies, due to lack of a stable fusion partner cell line for hybridoma development.
Sino Biological has developed a proprietary platform for efficient development of high affinity and high specificity rabbit monoclonal antibodies. We are the first company that is able to generate 2nd generation high-affinity rabbit Mab owning to our creative proprietary technology. By using recombinant protein based antigen for rabbit immunization, we can obtain rabbit monoclonal antibodies for ELISA assay as well as Western blotting, IHC, and flow cytometry applications. Sino Biological has successfully developed ELISA assays with rabbit monoclonal antibodies with pg assay sensitivity. Our rabbit monoclonal antibodies usually have binding affinity in the range of 1-100 picomole. Our rabbit monoclonal antibody platform is one of the kind in the industry and enables us to offer the most comprehensive one-stop custom antibody development services including mouse and rabbit monoclonal antibodies.