RTN4R / NOGOR Protein (His Tag)

Reticulon 4 receptor Protein Datasheet

RTN4R / NOGOR Protein Product Information

• Synonym: RTN4R, NOGOR
• Protein Construction: A DNA sequence encoding the human RTN4R (Q9BZR6) (Met1-Ser447) was expressed with a polyhistidine tag at the C-terminus.
• Source: Human
• Expression Host: Human Cells

RTN4R / NOGOR Protein QC Testing

• Purity: > 90% as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Cys 27
• Molecular Mass: The recombinant human RTN4R consists of 433 amino acids and predicts a molecular mass of 46.8 KDa. It migrates as an approximately 60 KDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS, pH 7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: RTN4R / NOGOR protein

RTN4R / NOGOR Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

RTN4R / NOGOR Protein Description

Reticulon 4 receptor (RTN4R), also known as Nogo-66 Receptor (NgR), is a glycosylphosphoinositol (GPI)-anchored protein that belongs to the Nogo recptor family including three members. Mouse RTN4R cDNA contains 10 LRP (Leucine-rich) repeats. RTN4R is expressed predominantly in neurons and their axons in the central nervous systems (CNS). As a receptor for myelin-derived proteins Nogo, myelin-associated glycoprotein (MAG), and myelin oligodendrocyte glycoprotein (OMG), RTN4R mediates axonal growth inhibition and may play a role in regulating axonal regeneration and plasticity in the adult CNS. It has been shown that RTN4R performs its inhibitory actions by interacting with the p75 neurotrophin receptor (p75NTR), a TNFRSF member also known for modulating the activities of the Trk family and for inducing apoptosis in neurons and oligodendrocytes. RTN4R may be proposed as a potential drug target for treatment of various neurological conditions such as spinal cord injury, CNS lesions, peripheral nerve injury, stroke and Alzheimer's disease (AD). Additionally, RTN4R may play a role in regulating the function of the gap junctions.

References

1. Wang X. et al., 2006, Ann Neurol. 60 (5): 540-9.
2. Wang YZ. et al., 2006, Neuroreport. 17 (6): 605-9.
3. Zhu HY. et al., 2007, Hum Pathol. 38 (3): 426-34.?
4. David S. et al., 2008, Trends Neurosci. 31 (5): 221-6.
5. Jiang W. et al., 2009, Transl Res. 154 (1): 40-8.
6. Zhang L. et al., 2009, J Neurosci, 9 (19): 6348-52.