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Human RTN4 Gene cDNA Clone / ORF Clone

Catalog Vector (Price) Quantity In Stock Operation
HG12261-G





  YES

*  pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors.
    Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

RTN4 cDNA Clone Datasheet

 

RTN4 cDNA Clone Product Information

Gene Bank Ref.ID: BC001035

cDNA Size:

600

Gene Synonym:

ASY; NSP; NOGO; NOGOC; RTN-X; NOGO-A; NSP-CL; Nogo-B; Nogo-C; RTN4-A; RTN4-C; RTN4-B1; RTN4-B2; NI220/250; Nbla00271; RTN4 Nbla10545

Source: Human

RTN4 cDNA Clone Description

cDNA Description:

ORF Clone of Homo sapiens reticulon 4 DNA.

Vector: Cloning Vector: pGEM-T Vector

Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

Sequence Description: Identical with the Gene Bank Ref.ID sequence.

RTN4 cDNA Clone Shipping

  1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
  2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

RTN4 cDNA Clone Usage Protocol

  1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
  2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H2O Ensure the filter is completely immersed in the H2O by briefly centrifuging the tube for 10 seconds.
  3. Transfer the tube to incubation at 95℃ for 15-30 min.
  4. At the end of the incubation pulse vortex, or gently tap the tube.
  5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

RTN4 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGACGGTC AGAAGAAAAA TTGGAAGGAC AAGGTTGTTG ACCTCCTGTA CTGGAGAGAC
61       ATTAAGAAGA CTGGAGTGGT GTTTGGTGCC AGCCTATTCC TGCTGCTTTC ATTGACAGTA
121      TTCAGCATTG TGAGCGTAAC AGCCTACATT GCCTTGGCCC TGCTCTCTGT GACCATCAGC
181      TTTAGGATAT ACAAGGGTGT GATCCAAGCT ATCCAGAAAT CAGATGAAGG CCACCCATTC
241      AGGGCATATC TGGAATCTGA AGTTGCTATA TCTGAGGAGT TGGTTCAGAA GTACAGTAAT
301      TCTGCTCTTG GTCATGTGAA CTGCACGATA AAGGAACTCA GGCGCCTCTT CTTAGTTGAT
361      GATTTAGTTG ATTCTCTGAA GTTTGCAGTG TTGATGTGGG TATTTACCTA TGTTGGTGCC
421      TTGTTTAATG GTCTGACACT ACTGATTTTG GCTCTCATTT CACTCTTCAG TGTTCCTGTT
481      ATTTATGAAC GGCATCAGGC ACAGATAGAT CATTATCTAG GACTTGCAAA TAAGAATGTT
541      AAAGATGCTA TGGCTAAAAT CCAAGCAAAA ATCCCTGGAT TGAAGCGCAA AGCTGAATGA

 

Quality Control:cDNA sequencing results is available upon request.

 

 

NOGO-A (RTN4) related areas, pathways, and other information