Human RSV glycoprotein G / RSV-G Long strain (subtype A) cDNA Clone / ORF Clone

Human RSV glycoprotein G / RSV-G Long strain (subtype A) cDNA Clone Datasheet

Human RSV glycoprotein G / RSV-G Long strain (subtype A) cDNA Clone Product Information

• cDNA Size: 910
• Gene Synonym: G, HRSVgp07
• Source: Human respiratory syncytial virus

Human RSV glycoprotein G / RSV-G Long strain (subtype A) cDNA Clone Description

• cDNA Description: ORF Clone of Human RSV glycoprotein G / RSV-G Long strain (subtype A) DNA.

  This cDNA clone has gone through customized codon optimization in order to obtain high level of protein expression in particular cell lines. Therefore, although the translated amino acid sequence is identical to the amino sequence on Gene Bank, the DNA sequence is different from that on Gene Bank.

• Vector: pGEM-T Vector

Human RSV glycoprotein G / RSV-G Long strain (subtype A) cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

Human RSV glycoprotein G / RSV-G Long strain (subtype A) cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

Human RSV glycoprotein G / RSV-G Long strain (subtype A) cDNA Clone Sequence and Quality Control

Sequence:

1        GGTACCATGA GTAAGAATAA GGATCAACGC ACGGCGAAGA CGCTCGAAAA GACGTGGGAC
61       ACGCTCAACC ACCTGCTGTT CATCTCCTCC GGGCTGTACA AGCTCAATCT CAAGTCCATC
121      GCCCAGATCA CGCTGAGCAT CCTCGCCATG ATCATAAGCA CGTCCCTTAT CATCACCGCG
181      ATCATCTTCA TCGCGTCCGC GAACCACAAG GTGACCCTCA CGACGGCCAT TATCCAGGAC
241      GCCACATCCC AAATCAAAAA CACCACGCCC ACCTACCTGA CCCAGGACCC GCAGCTGGGC
301      ATCAGCTTCT CCAACCTCAG CGAGATCACC TCCCAGACGA CGACCATCCT GGCCTCCACC
361      ACCCCGGGCG TGAAGAGCAA CCTGCAGCCG ACGACGGTCA AGACGAAGAA CACGACCACG
421      ACCCAAACGC AGCCAAGCAA GCCCACCACG AAGCAGCGAC AGAACAAGCC CCCCAACAAG
481      CCAAACAATG ACTTCCACTT TGAGGTGTTC AACTTTGTGC CATGTAGCAT CTGTAGCAAC
541      AACCCAACTT GTTGGGCTAT CTGTAAGAGG ATTCCAAACA AGAAGCCTGG CAAGAAGACC
601      ACCACCAAGC CAACCAAGAA GCCAACCTTC AAGACCACCA AGAAGGACCA TAAGCCACAG
661      ACGACCAAGC CGAAGGAGGT GCCCACCACG AAGCCGACGG AGGAGCCAAC GATCAACACA
721      ACGAAGACCA ACATCATCAC CACGCTGCTG ACGAACAACA CGACCGGGAA CCCCAAGCTC
781      ACGAGCCAGA TGGAGACGTT CCACAGCACC AGCTCCGAGG GCAACCTGAG CCCCTCCCAG
841      GTCAGCACGA CCAGTGAGCA CCCCAGCCAG CCCAGCAGTC CCCCCAACAC TACAAGGCAG
901      TAAATCTAGA

 

Quality Control:cDNA sequencing results is available upon request.