Human RSV Fusion glycoprotein / RSV-F Long strain (subtype A) ORF Expression Clone, FLAG-tagged

Human RSV Fusion glycoprotein / RSV-F Long strain (subtype A) cDNA Clone Datasheet

Human RSV Fusion glycoprotein / RSV-F Long strain (subtype A) cDNA Clone Product Information

• cDNA Size: 1752
• Gene Synonym: F, HRSVgp08
• Source: Human respiratory syncytial virus

Human RSV Fusion glycoprotein / RSV-F Long strain (subtype A) cDNA Clone Description

• cDNA Description: ORF Clone of Human RSV Fusion glycoprotein / RSV-F Long strain (subtype A) DNA.
• Vector: pCMV/hygro-FLAG
• FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG

Human RSV Fusion glycoprotein / RSV-F Long strain (subtype A) cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

Human RSV Fusion glycoprotein / RSV-F Long strain (subtype A) cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

Human RSV Fusion glycoprotein / RSV-F Long strain (subtype A) cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGAACTGC CGATTCTGAA AGCCAATGCC ATCACCACCA TCCTGGCAGC AGTGACCTTC
61       TGTTTTGCCT CTAGTCAAAA CATCACAGAG GAGTTCTACC AGAGCACTTG TTCTGCTGTG
121      AGCAAGGGCT ACCTGTCTGC CCTGAGGACA GGCTGGTACA CCTCTGTGAT TACCATTGAA
181      CTGAGCAACA TCAAGGAGAA CAAGTGTAAT GGCACAGATG CCAAGGTGAA ACTGATTAAG
241      CAGGAACTGG ACAAATACAA GAATGCTGTG ACAGAACTCC AACTTCTGAT GCAGAGCACA
301      CCTGCAGCCA ACAACAGGGC AAGGAGGGAA CTGCCAAGGT TTATGAACTA CACCCTGAAC
361      AATACCAAGA AGACCAATGT GACCCTGAGC AAGAAGAGGA AGAGGAGGTT CCTGGGCTTC
421      CTGCTGGGAG TGGGCTCTGC CATTGCCTCT GGAACGGCTG TGAGCAAGGT GCTGCACTTG
481      GAGGGAGAGG TGAACAAGAT TAAGTCTGCC CTGCTGAGCA CCAACAAGGC TGTGGTGTCC
541      CTGAGCAATG GAGTGTCTGT GCTGACCAGC AAGGTGCTGG ACCTGAAAAA CTACATTGAC
601      AAGCAACTTC TGCCAATTGT GAACAAGCAG TCCTGTAGAA TCAGCAACAT TGAGACAGTG
661      ATTGAGTTCC AACAGAAGAA CAACAGACTG TTGGAGATTA CCAGGGAGTT CTCTGTGAAT
721      GCTGGAGTGA CCACACCTGT GAGCACCTAT ATGCTGACCA ACTCTGAACT GCTGTCCCTG
781      ATAAATGATA TGCCAATCAC CAATGACCAG AAGAAACTGA TGAGTAACAA TGTCCAGATT
841      GTGAGACAAC AGTCCTACAG CATAATGAGT ATCATCAAGG AGGAGGTGCT GGCTTATGTG
901      GTCCAACTTC CACTCTATGG AGTGATTGAC ACACCATGTT GGAAACTGCA CACCAGCCCA
961      CTGTGTACCA CCAACACCAA GGAGGGCAGC AACATCTGTC TGACCAGGAC AGACAGGGGC
1021     TGGTATTGTG ACAATGCTGG CTCTGTGTCC TTCTTTCCAC AGGCTGAGAC TTGTAAGGTC
1081     CAGAGCAACA GGGTGTTCTG TGACACAATG AACTCCCTGA CCCTGCCATC TGAGGTAAAC
1141     CTGTGTAATG TGGACATCTT CAACCCAAAA TATGACTGTA AGATAATGAC CAGCAAGACA
1201     GATGTGTCCT CCTCTGTGAT TACCTCCCTG GGAGCCATTG TGTCCTGTTA TGGCAAGACC
1261     AAGTGTACTG CCAGCAACAA GAACAGGGGC ATCATCAAGA CCTTCAGCAA TGGCTGTGAC
1321     TATGCGAGCA ACAAGGGGGT GGACACAGTG TCTGTGGGCA ACACCCTCTA CTATGTGAAC
1381     AAGCAGGAGG GCAAGTCCCT CTATGTGAAG GGAGAACCAA TCATCAACTT CTATGACCCA
1441     CTGGTGTTTC CATCTGATGA GTTTGATGCC AGCATCAGCC AGGTGAATGA GAAGATAAAC
1501     CAGTCCCTGG CTTTCATCAG GAAGTCTGAT GAACTGCTGC ACCATGTGAA TGCTGGCAAG
1561     AGCACCACCA ACATAATGAT TACCACCATC ATAATCGTGA TCATCGTCAT CCTGCTGTCC
1621     CTCATCGCGG TCGGGCTCCT CCTGTATTGC AAGGCCAGGA GCACGCCGGT CACGCTGAGC
1681     AAGGACCAGC TGAGCGGCAT CAACAACATC GCGTTCAGCA ACGCT1GATTA CAAGGATGAC
1741     GACGATAAG2T AA

 

1. GCT was the nucleotide residue from the restriction site during plasmid construction,
which has no influence on protein expression.
2. FLAG Tag

 

 

Quality Control:cDNA sequencing results is available upon request.