Anti-RSPO1 Antibody

Cat: 11083-RP02
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Anti-RSPO1 Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-RSPO1 Antibody
Validated applications
WB,ELISA
Species reactivity
Reacts with: Human
Specificity
Human RSPO1
Immunogen
Recombinant Human RSPO1 protein (Catalog#11083-H08H)
Preparation
Produced in rabbits immunized with purified, recombinant Human RSPO1 / R-spondin-1 (rh RSPO1; Catalog#11083-H08H; NP_001033722.1; Met 1-Ala 263). RSPO1 specific IgG was purified by human RSPO1 affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-RSPO1 Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:1000  
ELISA 1:5000-1:10000  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-RSPO1 Antibody (Rabbit Polyclonal antibody) Images
Human R-Spondin 1/RSPO1 Western blot (WB) 13337

Anti-RSPO1 rabbit polyclonal antibody at 1:500 dilution

Lane A: HL-60 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:29 kDa

Observed band size:24 kDa

Anti-RSPO1 Antibody Alternative Names
Anti-CRISTIN3 Antibody;Anti-R-Spondin 1 Antibody;Anti-RSPO Antibody
RSPO1 Background Information

RSPO1 gene is a member of the R-spondin family. It encodes RSPO1 which is known as a secreted activator protein with two cystein-rich, furin-like domains and one thrombospondin type 1 domain. In mice, RSPO1 induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects. This protein is an activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. RSPO1 acts both in the canonical Wnt/beta-catenin-dependent pathway and in non-canonical Wnt signaling pathway, probably by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. It also acts as a ligand for frizzled FZD8 and LRP6.

Full Name
R-spondin 1
References
  • Kamata T, et al. (2004) R-spondin, a novel gene with thrombospondin type 1 domain, was expressed in the dorsal neural tube and affected in Wnts mutants. Biochim Biophys Acta. 1676(1):51-62.
  • Ota T, et al. (2004) Complete sequencing and characterization of 21,243 full-length human cDNAs. Nat Genet. 36(1):40-5.
  • Strausberg RL, et al. (2003) Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proc Natl Acad Sci. 99(26):16899-903.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-RSPO1 Antibody Rabbit Polyclonal ELISA,IHC-P 50316-T24
    Anti-RSPO1 Antibody Rabbit Polyclonal ELISA 50316-T16
    Anti-RSPO1 Antibody Rabbit Polyclonal ELISA 11083-T16
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