RRM2B / P53R2 Protein (His Tag)

Ribonucleotide reductase M2 B (TP53 inducible) Protein Datasheet

RRM2B / P53R2 Protein Product Information

• Synonym: DKFZp686M05248, MGC102856, MGC42116, MTDPS8A, MTDPS8B, P53R2
• Protein Construction: A DNA sequence encoding the human RRM2B (Q7LG56-1) (Met 1-Phe 351) was expressed, with a polyhistide tag at the N-terminus.
• Source: Human
• Expression Host: E. Coli

RRM2B / P53R2 Protein QC Testing

• Purity: > 92 % as determined by SDS-PAGE.
• Bio-activity: Measured by its ability to bind TP53 in a functional ELISA.
• Endotoxin: Please contact us for more information.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met
• Molecular Mass: The recombinant human RRM2B consisting of 366 amino acids and has a calculated molecular mass of 42.6 KDa. It migrates as a 43 kDa band in SDS-PAGE under reducing conditions as predicted.
• Formulation: Lyophilized from sterile PBS, 30%glycerol, pH 8.5
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: RRM2B / P53R2 protein

RRM2B / P53R2 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

RRM2B / P53R2 Protein Description

Ribonucleoside reductase subunit M2B(also known as RRM2B or p53R2) is an enzyme belonging to the iron-dependent ribonucleotide reductase (RNR) enzyme family which is essential for DNA synthesis. Ribonucleotide reductase (RNR) is an enzyme that catalyzes the formation of deoxyribonucleotides from ribonucleotides and plays a critical role in regulating the total rate of DNA synthesis so that DNA to cell mass is maintained at a constant ratio during cell division and DNA repair. Using in vivo 32P-orthophosphate labeling, it has been observed that 32P can be incorporated into RRM2B/p53R2 induced by UV irradiation , suggesting that p53R2 is a phosphorylated protein. It is hypothesized that p53R2 activity can be regulated at the posttranslational level in response to DNA damage. p53R2 has previously been shown to be essential for the maintenance of mtDNA copy number and its candidacy for tumor suppression has been evaluated in several mutational analyses of different cancer types. However, the contribution of p53R2 to the DNA damage response has been questioned because its transcriptional induction upon DNA damage is not rapid enough for prompt DNA repair. Instead, ATM-mediated phosphorylation has been suggested to regulate the DNA repair activity of p53R2 posttranslationally. In addition, a defect in p53R2 can induce a mild muscle disease of adult onset through disturbance of mitochondrial homeostasis but that this defect does not appear to be oncogenic.

References

1. Bourdon A. et al., 2007, Nature Genetics. 39: 776-80.
2. Tyynismaa H. et al., 2009, AJHG. 85 (2): 290-5.
3. Shaibani A. et al., 2009, Arch Neurol. 66 (8):1028-32.