Mouse RPE Gene cDNA Clone / ORF Clone

RPE cDNA Clone Datasheet

RPE cDNA Clone Product Information

• Gene Bank Ref.ID: NM_025683.2
• cDNA Size: 687
• Gene Synonym: 2810429B02Rik; 5730518J08Rik; Rpe
• Source: Mouse

RPE cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus ribulose-5-phosphate-3-epimerase DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

RPE cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

RPE cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

RPE cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCGTCGG GCTGCAAGAT CGGCCCGTCC ATCCTCAACA GCGACCTGGC CAACCTGGGG
61       GCCGAGTGCC TGCGCATGCT GGACTCGGGG GCCGACTACC TGCACCTGGA TGTAATGGAC
121      GGGCATTTTG TTCCCAATAT CACTTTTGGT CACCCTGTGG TTGAAAGCCT CCGAAAGCAG
181      CTAGGCCAGG ACCCTTTCTT TGATATGCAC ATGATGGTGT CTAGGCCAGA ACAGTGGGTA
241      AAACCAATGG CTGTGGCAGG TGCCAATCAA TATACCTTTC ATCTTGAGGC AACTGAGAAC
301      CCAGGGGCTT TGATTAAAGA CATCCGGGAG AATGGGATGA AGGTTGGCCT TGCCATCAAA
361      CCAGGAACTA CAGTTGAATA TTTGGCCCCA TGGGCTAATC AAATAGATAT GGCCTTGGTA
421      ATGACCGTAG AACCTGGGTT TGGAGGGCAG AAATTCATGG AAGACATGAT GCCCAAGGTT
481      CACTGGTTGA GGACCCAGTT TCCAACTTTG GATATAGAGG TCGATGGCGG AGTAGGTCCT
541      GACACTGTTC AGAAGTGTGC GGAGGCAGGA GCTAACATGA TTGTGTCTGG CAGTGCCATT
601      ATGAGGAGTG ATGACCCCAG AGCTGTTATC AACTTACTAA GAAACGTTTG CTCAGAAGCG
661      GCTCAGAAAC GTTCCCTTGA TCGATGA

 

Quality Control:cDNA sequencing results is available upon request.