Human RNF148 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13361-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

RNF148 cDNA Clone Datasheet

RNF148 cDNA Clone Product Information

Gene Bank Ref.ID:	BC029264
cDNA Size:	918
Gene Synonym:	RNF148
Source:	Human

RNF148 cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens ring finger protein 148 DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

RNF148 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

RNF148 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

RNF148 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAGCTTCC TTAGAATTAC CCCTTCGACG CATAGTTCTG TTTCATCTGG ACTTTTGAGG
61       CTTAGTATCT TTCTACTACT TAGCTTTCCT GACTCAAACG GAAAAGCCAT TTGGACAGCT
121      CACCTGAATA TAACATTTCA GGTTGGAAAT GAGATCACAT CGGAATTAGG AGAGAGTGGA
181      GTGTTCGGGA ATCATTCTCC TCTGGAAAGG GTGTCTGGTG TGGTGGCACT TCCTGAAGGA
241      TGGAATCAGA ATGCCTGTCA TCCTTTGACC AATTTCAGCA GGCCCAAACA GGCAGACTCA
301      TGGCTGGCCC TCATCGAACG TGGAGGCTGT ACTTTTACAC ATAAAATCAA CGTGGCAGCA
361      GAGAAGGGAG CAAATGGGGT GATCATCTAC AACTATCAAG GTACGGGCAG TAAAGTATTT
421      CCCATGTCTC ACCAGGGGAC GGAAAATATA GTCGCGGTGA TGATAAGCAA CCTGAAAGGC
481      ATGGAAATTT TGCACTCGAT TCAGAAAGGA GTCTATGTGA CAGTCATCAT TGAAGTGGGG
541      AGAATGCACA TGCAGTGGGT GAGCCATTAC ATCATGTATC TATTTACCTT CCTGGCTGCC
601      ACAATTGCCT ACTTTTACTT AGATTGCGTC TGGAGACTTA CACCTAGAGT GCCCAATTCT
661      TTCACCAGGA GGCGAAGTCA AATAAAGACA GATGTGAAGA AAGCTATTGA CCAGCTTCAA
721      CTGCGAGTTC TCAAAGAAGG GGATGAGGAA TTAGACCTAA ATGAAGACAA CTGTGTTGTT
781      TGCTTTGACA CATACAAACC CCAAGATGTA GTACGCATTT TAACTTGCAA ACATTTTTTC
841      CATAAGGCAT GCATTGACCC CTGGCTTTTA GCCCATAGGA CATGTCCCAT GTGCAAGTGT
901      GACATCCTGA AAACTTAA

Quality Control:cDNA sequencing results is available upon request.