Human RNF144B Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13702-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

RNF144B cDNA Clone Datasheet

RNF144B cDNA Clone Product Information

Gene Bank Ref.ID:	BC063311
cDNA Size:	909
Gene Synonym:	PIR2;IBRDC2;p53RFP;bA528A10.3;RNF144B
Source:	Human

RNF144B cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens ring finger 144B DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vector: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

RNF144B cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

RNF144B cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

RNF144B cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGGCTCAG CTGGTAGGCT CCACTATCTC GCCATGACTG CTGAAAATCC CACTCCTGGA
61       GACCTGGCTC CGGCCCCCCT CATCACTTGC AAACTCTGCC TGTGTGAGCA GTCTCTGGAC
121      AAGATGACCA CACTCCAGGA ATGCCAGTGC ATCTTTTGCA CAGCTTGCCT GAAACAGTAC
181      ATGCAGCTGG CAATCCGAGA AGGATGTGGG TCTCCCATCA CTTGCCCTGA CATGGTGTGC
241      CTAAACCACG GGACCCTGCA GGAAGCTGAG ATTGCCTGTT TGGTACCTGT GGACCAGTTT
301      CAACTTTATC AGAGGTTAAA ATTTGAAAGA GAAGTTCATC TGGACCCCTA CCGAACATGG
361      TGTCCTGTTG CAGACTGTCA GACAGTGTGC CCTGTTGCCT CGAGTGACCC AGGACAGCCT
421      GTGCTGGTGG AATGCCCTTC TTGCCACCTG AAATTCTGCT CGTGTTGCAA GGATGCTTGG
481      CATGCAGAGG TCTCCTGTAG AGACAGTCAG CCTATTGTCC TGCCAACAGA GCACCGAGCC
541      CTCTTTGGGA CAGATGCAGA AGCCCCCATT AAGCAGTGCC CAGTTTGCCG GGTTTATATC
601      GAACGCAATG AAGGCTGCGC TCAGATGATG TGCAAAAACT GCAAGCATAC ATTTTGCTGG
661      TACTGCCTTC AGAACTTGGA TAATGACATT TTCCTCAGAC ATTATGACAA AGGGCCATGC
721      AGGAATAAAC TTGGCCACTC AAGAGCATCA GTGATGTGGA ACCGAACACA GGTGGTGGGG
781      ATTCTCGTAG GCTTGGGCAT CATTGCCTTG GTTACTTCCC CCTTACTCCT GGCCTCCCCA
841      TGTATAATCT GTTGTGTCTG CAAGTCCTGT CGGGGCAAGA AGAAAAAGCA CGACCCATCC
901      ACAACCTAA

Quality Control:cDNA sequencing results is available upon request.