Human RNF133 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13377-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

RNF133 cDNA Clone Datasheet

RNF133 cDNA Clone Product Information

Gene Bank Ref.ID:	BC022038
cDNA Size:	1131
Gene Synonym:	RNF133
Source:	Human

RNF133 cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens ring finger protein 133 DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

RNF133 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

RNF133 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

RNF133 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCATCTAC TCAAGGTTGG CACTTGGAGA AACAACACTG CCTCTTCCTG GCTTATGAAG
61       TTCAGTGTTC TTTGGCTTGT TAGTCAGAAC TGTTGCAGAG CAAGTGTTGT TTGGATGGCT
121      TATATGAACA TATCATTTCA TGTTGGGAAT CATGTGTTGT CAGAGTTGGG AGAGACTGGA
181      GTCTTTGGAA GAAGCTCCAC TTTGAAGAGA GTGGCAGGAG TTATAGTGCC ACCAGAGGGA
241      AAAATCCAAA ATGCATGTAA TCCCAATACC ATTTTCAGCC GATCAAAGTA CTCAGAGACC
301      TGGCTTGCAC TTATTGAACG GGGAGGTTGT ACCTTCACAC AGAAAATTAA AGTGGCAACT
361      GAGAAGGGAG CCAGTGGAGT GATCATCTAT AACGTTCCAG GTACTGGCAA CCAGGTGTTC
421      CCCATGTTTC ATCAGGCATT TGAAGATGTC GTTGTGGTTA TGATTGGTAA CTTAAAAGGC
481      ACGGAAATTT TCCATTTAAT TAAGAAGGGA GTTCTCATTA CAGCCGTGGT TGAGGTGGGG
541      AGAAAGCACA TCATCTGGAT GAATCACTAT TTGGTCTCTT TTGTGATTGT CACAACTGCT
601      ACCTTAGCAT ATTTCATCTT TTATCACATT CATAGACTTT GTTTAGCAAG GATTCAGAAC
661      CGGAGATGGC AGCGATTAAC AACAGATCTT CAGAACACAT TTGGACAACT CCAACTTCGA
721      GTAGTAAAAG AGGGGGATGA AGAAATAAAT CCAAATGGGG ATAGCTGCGT AATTTGCTTT
781      GAACGCTATA AGCCTAATGA CATAGTTCGT ATTCTGACTT GTAAACATTT TTTCCACAAG
841      AATTGCATTG ACCCCTGGAT TTTACCCCAT GGGACATGCC CCATTTGCAA ATGTGATATT
901      CTTAAAGTTT TGGGGATTCA AGTGGTTGTT GAAAATGGAA CAGAACCTTT GCAAGTTCTA
961      ATGTCAAATG AACTGCCTGA AACCTTATCA CCTAGTGAAG AGGAGACAAA TAATGAAGTT
1021     TCTCCTGCAG GAACCTCAGA TAAAGTAATC CATGTGGAGG AGAACCCTAC TTCTCAGAAT
1081     AATGACATCC AGCCTCATTC AGTAGTGGAA GATGTTCATC CTTCACCTTG A

Quality Control:cDNA sequencing results is available upon request.