Mouse RNASEL Gene cDNA Clone / ORF Clone

RNASEL cDNA Clone Datasheet

RNASEL cDNA Clone Product Information

• Gene Bank Ref.ID: NM_011882.2
• cDNA Size: 2208
• Gene Synonym: E230029I04Rik; Rnasel
• Source: Mouse

RNASEL cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus ribonuclease L (2', 5'-oligoisoadenylate synthetase-dependent)S DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref. ID sequence.

RNASEL cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

RNASEL cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

RNASEL cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGAGACCC CGGATTATAA CACACCTCAG GGTGGAACCC CATCAGCGGG AAGTCAGAGG
61       ACCGTTGTCG AAGATGATTC TTCGTTGATC AAAGCTGTTC AGAAGGGAGA TGTTGTCAGG
121      GTCCAGCAAT TGTTAGAAAA AGGGGCTGAT GCCAATGCCT GTGAAGACAC CTGGGGCTGG
181      ACACCTTTGC ACAACGCAGT GCAAGCTGGC AGGGTAGACA TTGTGAACCT CCTGCTTAGT
241      CATGGTGCTG ACCCTCATCG GAGGAAGAAG AATGGGGCCA CCCCCTTCAT CATTGCTGGG
301      ATCCAGGGAG ATGTGAAACT GCTCGAGATT CTCCTCTCTT GTGGTGCAGA CGTCAATGAG
361      TGTGACGAGA ACGGATTCAC GGCTTTCATG GAAGCTGCTG AGCGTGGTAA CGCTGAAGCC
421      TTAAGATTCC TTTTTGCTAA GGGAGCCAAT GTGAATTTGC GACGACAGAC AACGAAGGAC
481      AAAAGGCGAT TGAAGCAAGG AGGCGCCACA GCTCTCATGA GCGCTGCTGA GAAGGGCCAC
541      CTGGAAGTCC TGAGAATTCT CCTCAATGAC ATGAAGGCAG AAGTCGATGC TCGGGACAAC
601      ATGGGCAGAA ATGCCCTGAT CCGTACTCTG CTGAACTGGG ATTGTGAAAA TGTGGAGGAG
661      ATTACTTCAA TCCTGATTCA GCACGGGGCT GATGTTAACG TGAGAGGAGA AAGAGGGAAA
721      ACACCCCTCA TCGCAGCAGT GGAGAGGAAG CACACAGGCT TGGTGCAGAT GCTCCTGAGT
781      CGGGAAGGCA TAAACATAGA TGCCAGGGAT AACGAGGGCA AGACAGCTCT GCTAATTGCT
841      GTTGATAAAC AACTGAAGGA AATTGTCCAG TTGCTTCTTG AAAAGGGAGC TGATAAGTGT
901      GACGATCTTG TTTGGATAGC CAGGAGGAAT CATGACTATC ACCTTGTAAA GCTTCTCCTC
961      CCTTATGTAG CTAATCCTGA CACCGACCCT CCTGCTGGAG ACTGGTCGCC TCACAGTTCA
1021     CGTTGGGGGA CAGCCTTGAA AAGCCTCCAC AGTATGACTC GACCCATGAT TGGCAAACTC
1081     AAGATCTTCA TTCATGATGA CTATAAAATT GCTGGCACTT CCGAAGGGGC TGTCTACCTA
1141     GGGATCTATG ACAATCGAGA AGTGGCTGTG AAGGTCTTCC GTGAGAATAG CCCACGTGGA
1201     TGTAAGGAAG TCTCTTGTCT GCGGGACTGC GGTGACCACA GTAACTTAGT GGCTTTCTAT
1261     GGAAGAGAGG ACGATAAGGG CTGTTTATAT GTGTGTGTGT CCCTGTGTGA GTGGACACTG
1321     GAAGAGTTCC TGAGGTTGCC CAGAGAGGAA CCTGTGGAGA ACGGGGAAGA TAAGTTTGCC
1381     CACAGCATCC TATTATCTAT ATTTGAGGGT GTTCAAAAAC TACACTTGCA TGGATATTCC
1441     CATCAGGACC TGCAACCACA AAACATCTTA ATAGATTCCA AGAAAGCTGT CCGGCTGGCA
1501     GATTTTGATC AGAGCATCCG ATGGATGGGA GAGTCACAGA TGGTCAGGAG AGACTTGGAG
1561     GATCTTGGAC GGCTGGTTCT CTACGTGGTA ATGAAAGGTG AGATCCCCTT TGAGACACTA
1621     AAGACTCAGA ATGATGAAGT GCTGCTTACA ATGTCTCCAG ATGAGGAGAC TAAGGACCTC
1681     ATTCATTGCC TGTTTTCTCC TGGAGAAAAT GTCAAGAACT GCCTGGTAGA CCTGCTTGGC
1741     CATCCTTTCT TTTGGACTTG GGAGAACCGC TATAGAACAC TCCGGAATGT GGGAAATGAA
1801     TCTGACATCA AAGTACGGAA ATGTAAAAGT GATCTTCTCA GACTACTGCA GCATCAGACA
1861     CTTGAGCCTC CCAGAAGCTT TGACCAGTGG ACATCTAAGA TCGACAAAAA TGTTATGGAT
1921     GAAATGAATC ATTTCTACGA AAAGAGAAAA AAAAACCCTT ATCAGGATAC TGTAGGTGAT
1981     CTGCTGAAGT TTATTCGGAA TATAGGCGAA CACATCAATG AGGAAAAAAA GCGGGGAATG
2041     AAGGAGATAC TTGGAGATCC TTCTCGTTAT TTTCAGGAGA CATTTCCAGA TCTTGTAATC
2101     TATATCTACA AGAAACTAAA GGAAACAGAA TACAGAAAAC ACTTCCCACA GCCTCCACCA
2161     CGCCTCAGCG TTCCAGAGGC AGTGGGACCA GGGGGCATAC AGAGCTGA

 

Quality Control:cDNA sequencing results is available upon request.