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Human RNASE1 Gene cDNA Clone / ORF Clone

Catalog Vector (Price) Quantity In Stock Operation
HG13468-G





  YES

*  pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors.
    Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

RNASE1 cDNA Clone Datasheet

 

RNASE1 cDNA Clone Product Information

Gene Bank Ref.ID: BC005324

cDNA Size:

471

Gene Synonym:

RIB1; RNS1; RNASE1

Source: Human

RNASE1 cDNA Clone Description

cDNA Description:

ORF Clone of Homo sapiens ribonuclease, RNase A family, 1 (pancreatic) DNA.

Vector: Cloning Vector: pGEM-T Vector

Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

Sequence Description: Identical with the Gene Bank Ref.ID sequence.

RNASE1 cDNA Clone Shipping

  1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
  2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

RNASE1 cDNA Clone Usage Protocol

  1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
  2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H2O Ensure the filter is completely immersed in the H2O by briefly centrifuging the tube for 10 seconds.
  3. Transfer the tube to incubation at 95℃ for 15-30 min.
  4. At the end of the incubation pulse vortex, or gently tap the tube.
  5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

RNASE1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCTCTGG AGAAGTCTCT TGTCCGGCTC CTTCTGCTTG TCCTGATACT GCTGGTGCTG
61       GGCTGGGTCC AGCCTTCCCT GGGCAAGGAA TCCCGGGCCA AGAAATTCCA GCGGCAGCAT
121      ATGGACTCAG ACAGTTCCCC CAGCAGCAGC TCCACCTACT GTAACCAAAT GATGAGGCGC
181      CGGAATATGA CACAGGGGCG GTGCAAACCA GTGAACACCT TTGTGCACGA GCCCCTGGTA
241      GATGTCCAGA ATGTCTGTTT CCAGGAAAAG GTCACCTGCA AGAACGGGCA GGGCAACTGC
301      TACAAGAGCA ACTCCAGCAT GCACATCACA GACTGCCGCC TGACAAACGG CTCCAGGTAC
361      CCCAACTGTG CATACCGGAC CAGCCCGAAG GAGAGACACA TCATTGTGGC CTGTGAAGGG
421      AGCCCATATG TGCCAGTCCA CTTTGATGCT TCTGTGGAGG ACTCTACCTA A

 

Quality Control:cDNA sequencing results is available upon request.

 

 

RNase A (Ribonuclease A, RNASE1) related areas, pathways, and other information