Mouse RIPK1 ORF Expression Clone, untagged

RIPK1 cDNA Clone Datasheet

RIPK1 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_009068.3
• cDNA Size: 1971
• Gene Synonym: RIP; Rinp; Rip1; D330015H01Rik; Ripk1
• Source: Mouse

RIPK1 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus receptor (TNFRSF)-interacting serine-threonine kinase 1 DNA.
• Vector: pCMV/hygro
• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 1418 C/T resulting in the amino acid Thr substitution by Ile.

RIPK1 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

RIPK1 cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

RIPK1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCAACCAG ACATGTCCTT GGACAATATT AAGATGGCAT CCAGTGACCT GCTGGAGAAG
61       ACAGACCTAG ACAGCGGAGG CTTCGGGAAG GTGTCCTTGT GTTACCACAG AAGCCATGGA
121      TTTGTCATCC TGAAAAAAGT ATACACAGGG CCCAACCGCG CTGAGTACAA TGAGGTTCTC
181      TTGGAAGAGG GGAAGATGAT GCACAGACTG AGACACAGTC GAGTGGTGAA GCTACTGGGC
241      ATCATCATAG AAGAAGGGAA CTATTCGCTG GTGATGGAGT ACATGGAGAA GGGCAACCTG
301      ATGCACGTGC TAAAGACCCA GATAGATGTC CCACTTTCAT TGAAAGGAAG GATAATCGTG
361      GAGGCCATAG AAGGCATGTG CTACTTACAT GACAAAGGTG TGATACACAA GGACCTGAAG
421      CCTGAGAATA TCCTCGTTGA TCGTGACTTT CACATTAAGA TAGCCGATCT TGGTGTGGCT
481      TCCTTTAAGA CATGGAGCAA ACTGACTAAG GAGAAAGACA ACAAGCAGAA AGAAGTGAGC
541      AGCACCACTA AGAAGAACAA TGGTGGTACC CTTTACTACA TGGCACCCGA ACACCTGAAT
601      GACATCAATG CAAAGCCCAC GGAGAAGTCG GACGTGTACA GCTTTGGCAT TGTCCTTTGG
661      GCAATATTTG CAAAAAAGGA GCCCTATGAG AATGTCATCT GTACTGAGCA GTTCGTGATC
721      TGCATAAAAT CTGGGAACAG GCCAAATGTA GAGGAAATCC TTGAGTACTG TCCAAGGGAG
781      ATCATCAGCC TCATGGAGCG GTGCTGGCAG GCGATCCCAG AAGACAGGCC AACATTTCTT
841      GGCATTGAAG AAGAATTTAG GCCTTTTTAC TTAAGTCATT TTGAAGAATA TGTAGAAGAG
901      GATGTGGCAA GTTTAAAGAA AGAGTATCCA GATCAAAGCC CAGTGCTGCA GAGAATGTTT
961      TCACTGCAGC ATGACTGTGT ACCCTTACCT CCGAGCAGGT CAAATTCAGA ACAACCTGGA
1021     TCGCTGCACA GTTCCCAGGG GCTCCAGATG GGTCCTGTGG AGGAGTCCTG GTTTTCTTCC
1081     TCCCCAGAGT ACCCACAGGA CGAGAATGAT CGCAGTGTGC AGGCTAAGCT GCAAGAGGAA
1141     GCCAGCTATC ATGCTTTTGG AATATTTGCA GAGAAACAGA CAAAACCGCA GCCAAGGCAG
1201     AATGAGGCTT ACAACAGAGA GGAGGAAAGG AAACGAAGGG TCTCTCATGA CCCCTTTGCA
1261     CAGCAGAGAG CTCGTGAGAA TATTAAGAGT GCAGGAGCAA GAGGTCATTC TGATCCCAGC
1321     ACAACGAGTC GTGGAATTGC AGTGCAACAG CTGTCATGGC CAGCCACCCA AACAGTTTGG
1381     AACAATGGAT TGTATAATCA GCATGGATTT GGAACTATAG GTACAGGAGT TTGGTATCCG
1441     CCAAATCTAA GCCAAATGTA TAGTACTTAT AAAACTCCAG TGCCAGAGAC CAACATACCG
1501     GGAAGCACAC CCACCATGCC ATACTTCTCT GGGCCAGTAG CAGATGACCT CATAAAATAT
1561     ACTATATTCA ATAGTTCTGG TATTCAGATT GGAAACCACA ATTATATGGA TGTTGGACTG
1621     AATTCACAAC CACCAAACAA TACTTGCAAA GAAGAGTCGA CTTCCAGACA CCAAGCCATC
1681     TTTGATAACA CCACTAGTCT GACTGATGAA CACCTGAACC CTATCAGGGA AAACCTGGGA
1741     AGGCAGTGGA AAAACTGTGC CCGCAAGCTG GGCTTCACTG AGTCTCAGAT CGATGAAATC
1801     GACCATGACT ATGAAAGAGA TGGACTGAAA GAGAAAGTTT ACCAAATGCT TCAGAAGTGG
1861     CTGATGCGGG AAGGCACCAA AGGGGCCACA GTGGGAAAGT TGGCCCAGGC ACTTCACCAA
1921     TGTTGCAGGA TAGACCTGCT GAACCACTTG ATTCGTGCCA GCCAGAGCTA A

 

 

Quality Control:cDNA sequencing results is available upon request.