+86-400-890-9989
Product Catalog
Research Topics
Your Position: Home
> Gene Products > Human cDNA Clones > Human RIPK1 ORF Expression Clone, FLAG-tagged Human RIPK1 ORF Expression Clone, FLAG-tagged
| Catalog | Vector (Price) | Quantity | In Stock | Operation |
| HG11268-M-F |
|
YES |  |
|
|
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. |
 |
|||
RIPK1 cDNA Clone Datasheet
RIPK1 cDNA Clone Product Information
|
Gene Bank Ref.ID: |
NM_003804.3 |
|
cDNA Size: |
2043 |
| Gene Synonym: |
RIP; RIP1; FLJ39204; RIPK1 |
| Source: | Human |
RIPK1 cDNA Clone Description
|
cDNA Description: |
ORF Clone of Homo sapiens receptor (TNFRSF)-interacting serine-threonine kinase 1 DNA. |
| Vector: | |
| FLAG Tag Sequence: | GATTACAAGGATGACGACGATAAG |
| Sequence Description: | Identical with the Gene Bank Ref. ID sequence except for three point mutations: 84 T/C, 381 C/T, 910 T/C not causing the amino acid variation. |
RIPK1 cDNA Clone Shipping
- 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
- It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.
RIPK1 cDNA Clone Usage Protocol
- Cut along the circle edge of the spot (arrow points to) to get the filter paper.
- Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H2O Ensure the filter is completely immersed in the H2O by briefly centrifuging the tube for 10 seconds.
- Transfer the tube to incubation at 95℃ for 15-30 min.
- At the end of the incubation pulse vortex, or gently tap the tube.
- Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.
RIPK1 cDNA Clone Sequence and Quality Control
Sequence:
which has no influence on protein expression.
2. FLAG Tag
1 ATGCAACCAG ACATGTCCTT GAATGTCATT AAGATGAAAT CCAGTGACTT CCTGGAGAGT 61 GCAGAACTGG ACAGCGGAGG CTTCGGGAAG GTGTCTCTGT GTTTCCACAG AACCCAGGGA 121 CTCATGATCA TGAAAACAGT GTACAAGGGG CCCAACTGCA TTGAGCACAA CGAGGCCCTC 181 TTGGAGGAGG CGAAGATGAT GAACAGACTG AGACACAGCC GGGTGGTGAA GCTCCTGGGC 241 GTCATCATAG AGGAAGGGAA GTACTCCCTG GTGATGGAGT ACATGGAGAA GGGCAACCTG 301 ATGCACGTGC TGAAAGCCGA GATGAGTACT CCGCTTTCTG TAAAAGGAAG GATAATTTTG 361 GAAATCATTG AAGGAATGTG TTACTTACAT GGAAAAGGCG TGATACACAA GGACCTGAAG 421 CCTGAAAATA TCCTTGTTGA TAATGACTTC CACATTAAGA TCGCAGACCT CGGCCTTGCC 481 TCCTTTAAGA TGTGGAGCAA ACTGAATAAT GAAGAGCACA ATGAGCTGAG GGAAGTGGAC 541 GGCACCGCTA AGAAGAATGG CGGCACCCTC TACTACATGG CGCCCGAGCA CCTGAATGAC 601 GTCAACGCAA AGCCCACAGA GAAGTCGGAT GTGTACAGCT TTGCTGTAGT ACTCTGGGCG 661 ATATTTGCAA ATAAGGAGCC ATATGAAAAT GCTATCTGTG AGCAGCAGTT GATAATGTGC 721 ATAAAATCTG GGAACAGGCC AGATGTGGAT GACATCACTG AGTACTGCCC AAGAGAAATT 781 ATCAGTCTCA TGAAGCTCTG CTGGGAAGCG AATCCGGAAG CTCGGCCGAC ATTTCCTGGC 841 ATTGAAGAAA AATTTAGGCC TTTTTATTTA AGTCAATTAG AAGAAAGTGT AGAAGAGGAC 901 GTGAAGAGTC TAAAGAAAGA GTATTCAAAC GAAAATGCAG TTGTGAAGAG AATGCAGTCT 961 CTTCAACTTG ATTGTGTGGC AGTACCTTCA AGCCGGTCAA ATTCAGCCAC AGAACAGCCT 1021 GGTTCACTGC ACAGTTCCCA GGGACTTGGG ATGGGTCCTG TGGAGGAGTC CTGGTTTGCT 1081 CCTTCCCTGG AGCACCCACA AGAAGAGAAT GAGCCCAGCC TGCAGAGTAA ACTCCAAGAC 1141 GAAGCCAACT ACCATCTTTA TGGCAGCCGC ATGGACAGGC AGACGAAACA GCAGCCCAGA 1201 CAGAATGTGG CTTACAACAG AGAGGAGGAA AGGAGACGCA GGGTCTCCCA TGACCCTTTT 1261 GCACAGCAAA GACCTTACGA GAATTTTCAG AATACAGAGG GAAAAGGCAC TGCTTATTCC 1321 AGTGCAGCCA GTCATGGTAA TGCAGTGCAC CAGCCCTCAG GGCTCACCAG CCAACCTCAA 1381 GTACTGTATC AGAACAATGG ATTATATAGC TCACATGGCT TTGGAACAAG ACCACTGGAT 1441 CCAGGAACAG CAGGTCCCAG AGTTTGGTAC AGGCCAATTC CAAGTCATAT GCCTAGTCTG 1501 CATAATATCC CAGTGCCTGA GACCAACTAT CTAGGAAATA CACCCACCAT GCCATTCAGC 1561 TCCTTGCCAC CAACAGATGA ATCTATAAAA TATACCATAT ACAATAGTAC TGGCATTCAG 1621 ATTGGAGCCT ACAATTATAT GGAGATTGGT GGGACGAGTT CATCACTACT AGACAGCACA 1681 AATACGAACT TCAAAGAAGA GCCAGCTGCT AAGTACCAAG CTATCTTTGA TAATACCACT 1741 AGTCTGACGG ATAAACACCT GGACCCAATC AGGGAAAATC TGGGAAAGCA CTGGAAAAAC 1801 TGTGCCCGTA AACTGGGCTT CACACAGTCT CAGATTGATG AAATTGACCA TGACTATGAG 1861 CGAGATGGAC TGAAAGAAAA GGTTTACCAG ATGCTCCAAA AGTGGGTGAT GAGGGAAGGC 1921 ATAAAGGGAG CCACGGTGGG GAAGCTGGCC CAGGCGCTCC ACCAGTGTTC CAGGATCGAC 1981 CTTCTGAGCA GCTTGATTTA CGTCAGCCAG AACGCT1GATT ACAAGGATGA CGACGATAAG2 2041 TAA
1. GCT was the nucleotide residue from the restriction site during plasmid construction,
which has no influence on protein expression.
2. FLAG Tag
Quality Control:cDNA sequencing results is available upon request.
