RFK Protein, Human, Recombinant (His Tag)

Cat: 13881-H07E
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All RFK Reagents
RFK Protein, Human, Recombinant (His Tag) Product Information
Purity
> 90 % as determined by SDS-PAGE
Endotoxin
Please contact us for more information.
Activity
Kinase activity untested
Protein Construction
A DNA sequence encoding the mature form of human RFK (Q969G6) (Met1-His155) was expressed with a polyhistide tag at the N-terminus.
Reference#
Expressed Host
E. coli
Species
Human
Predicted N Terminal
His
Molecule Mass
The recombinant human RFK consists of 170 amino acids and predicts a molecular mass of 19.5 KDa. It migrates as an approximately 20 KDa band in SDS-PAGE under reducing conditions.
Formulation
Supplied as sterile 20mM Tris, 10% glycerol, pH 8.0
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Shipping
Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.
Shipment made at ambient temperature may seriously affect the activity of the ordered products.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -70℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
RFK Protein, Human, Recombinant (His Tag) in SDS-PAGE
Human Flavokinase / RFK Protein 10470
RFK Protein, Human, Recombinant (His Tag) Alternative Names
RIFK Protein, Human
RFK Background Information

Flavokinase is a member of the transferases family, specifically those transferring phosphorus-containing groups (phosphotransferases) with an alcohol group as acceptor. Flavokinase is an essential enzyme that catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN), an obligatory step in vitamin B2 utilization and flavin cofactor synthesis. It has been proposed that TNF, through the activation of the flavokinase gene, enhances the incorporation of FAD in NADPH oxidase enzymes, which is a critical step for the assembly and activation of NADPH oxidase.

Full Name
riboflavin kinase
References
  • Hirano G, et al. (2011) Involvement of riboflavin kinase expression in cellular sensitivity against cisplatin. Int J Oncol. 38(4):893-902.
  • Danielsen JM, et al. (2011) Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level. Mol Cell Proteomics. 10(3):M110.003590.
  • Danielsen JM, et al. (2011) Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level. Mol Cell Proteomics. 10(3):M110.003590.
  • Other RFK Proteins

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