Rat RELT Gene cDNA Clone / ORF Clone

RELT cDNA Clone Datasheet

RELT cDNA Clone Product Information

• Gene Bank Ref.ID: NM_001108495.1
• cDNA Size: 1284
• Gene Synonym: Tnfrsf19l, Relt
• Source: Rat

RELT cDNA Clone Description

• cDNA Description: ORF Clone of Rattus norvegicus RELT tumor necrosis factor receptor DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 522 G/C not causing the amino acid variation.

RELT cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

RELT cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

RELT cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAAGCGGA CCTTGCTGTG CTGGCCCCTG TCTTGCCTCT TTGTGCTGCT GCCCTGGCCC
61       CTGGCCACTC CAACACCCAT AACTCCTTGG CTGTGTCCCC CTGGCACGGA GCCTGACCCG
121      GATCCAGGGC AGGGCACATT ATGCAGAACT TGCCCTCCAG GCACCTTTTC AGCCTCACTG
181      GACTCCTATC CATGCCAGCC TCATTACCGC TGCGGCCTTC AAAAGAGGTT GGAGGCCCAG
241      GCTGGCACAG CAACTCATGA TACAGTGTGT GGAGACTGCC AGCGTGGGTG GTTTGGGCCA
301      CAGGGAGTTC CTCATGTTCC GTGTCAGCCA TGTTCCAAGG CAACTCCAAG TACCAGTGAC
361      TGTGATGGCC GGCGGGCCCG GCGTGGTGTC GAAGTGGCAG CAGGTGCTAA TAGCAATGGT
421      GAACTCCGGC AGCCTGGGAA TGGCACGCGG GCAGGCGGTC CTGAGGAGAC GGCTGCTCAG
481      TATGCAGTGA TTGCCATCGT TCCTGTCTTT TGTCTCATGG GCCTTCTGGG CATTCTGGTG
541      TGCAACCTGC TCAAGCGGAA GGGCTACCAC TGCACAGCCC AGAAGGAGGT CGGGCCCAGC
601      CCTGGCGGAG GAGGCAGTGG GATCAATCCC GCCTATAGGA CAGAGGATGC CAATGAGGAC
661      ACCATTGGAG TCCTGGTGCG CCTAATCACA GAGAAGAAAG AAAATGCAGC GGCCCTGGAG
721      GAGCTGTTGA AAGAATATCA CAGCAAACAG CTGGTACAGA CAAGCCACAG GCCTGTACCC
781      AGGCTGCTGC CGGCCTCCCC CAGCACACCA CACGTCTGCC CACACCATCA CCACCTCCAC
841      ACTGTGCAGG GCCTGGCCTC CCTCTCTGGC CCCTGCTGCT CCCGTTGTAG CCAGAAGTGG
901      CCAGAGGTGC TGCTGTCACC CGAGGCAGCA GCTGCCACCA CTCCTGCTCC CACCCTCCTG
961      CCTTCTACAT CCAGGGCTCC CAAGGCTAGT GCCAAGCCAG GACGTCAGGG CGAGATTACC
1021     ATCTTGTCTG TGGGCAGGTT CCGTGTGGCT CGCATTCCTG AGCAGCGGAC CAGTTCGTTG
1081     TTATCTGAGG TGAAGACTAT CACAGAGGCT GGGCCTTCAG AGAGCGATCT TCCTGAGTCC
1141     CCACAGCCTG GCCTTCCCCC CGAGCAGCGG GCACTGCTGG GAAGTAGTGG GAGTCATGCT
1201     AAGTGGTTGA AGCCCCCAGC AGAGAACAAG GCTGAGGAGA ACCGCTATGT GGTCCGGCTA
1261     AGTGAAAGCA ACCTGGTCAT CTGA

 

Quality Control:cDNA sequencing results is available upon request.