Rat REG4 Gene cDNA Clone / ORF Clone

REG4 cDNA Clone Datasheet

REG4 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_001004096.1
• cDNA Size: 474
• Gene Synonym: Reg4
• Source: Rat

REG4 cDNA Clone Description

• cDNA Description: ORF Clone of Rattus norvegicus regenerating islet-derived family, member 4 DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

REG4 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

REG4 cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

REG4 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCTTCCA AATGCGTGAG GCTACTCCTT CTGCTGAGCT GGGTAGCTGG CCCTGAAGTC
61       CTGAGTGATA TCTTGAGACC CAGCTGTGCT TCTGGATGGT TCAACTATAG GTCCCACTGT
121      TATGGGTACT TCCGGAAGCT AAGAAACTGG TCTCATGCTG AGCTGGAATG CCAGTCATAT
181      GGAAACGGAT CCCATCTGGC ATCTGTGTTG AATCCAAAGG AAGCCAGCGT CATATCAAAG
241      TACATAACTG GCTATCAGAG AAGCCTGCCT GTGTGGATTG GCCTGCATGA CCCACAGAAG
301      AATGCATCAT GGCAGTGGAT TGATGGGTCT ACAAACCAGT ACAGACCCTG GAGCCCCAGG
361      ACAAAAAGTG AAGCCAGGCA CTGCACTGAG ATGAACCCCA AGGATAAATT TTTAACTTGG
421      AACAAAAATG GATGCACCAA GCGCCAACAC TTCCTGTGCA AGTACAGGCC ATAG

 

Quality Control:cDNA sequencing results is available upon request.