Mouse REG4 Gene cDNA Clone / ORF Clone

REG4 cDNA Clone Datasheet

REG4 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_026328.1
• cDNA Size: 474
• Gene Synonym: GISP; RELP; 2010002L15Rik; Reg4
• Source: Mouse

REG4 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus regenerating islet-derived family, member 4 DNA.
• Vector: Vector pMD18-T Simple
• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

REG4 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

REG4 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

REG4 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCTTCCA AAGGCGTGCG GCTACTCTTA CTGCTGAGCT GGGTAGCTGG CCCCGAAGTC
61       CTGAGCGATA TCTTGAGACC CAGCTGTGCC CCAGGATGGT TTTACTATAG GTCCCACTGC
121      TATGGGTACT TCCGGAAGCT AAGAAACTGG TCTCATGCTG AGCTGGAGTG TCAGTCATAT
181      GGAAATGGAT CCCATCTGGC ATCTGTCTTG AATCAAAAGG AAGCCAGTGT CATATCAAAG
241      TACATAACTG GCTATCAGAG AAACCTGCCT GTGTGGATTG GCCTGCATGA CCCACAAAAG
301      AAGCAATTAT GGCAGTGGAC TGATGGGTCT ACAAACCTGT ACAGACGCTG GAATCCCAGG
361      ACAAAGAGTG AAGCCAGGCA TTGCGCTGAG ATGAACCCCA AGGATAAATT CTTAACTTGG
421      AACAAAAATG GATGTGCCAA CCGCCAACAC TTCCTGTGCA AGTATAAGAC ATAG

 

Quality Control:cDNA sequencing results is available upon request.