Mouse REG4 / GISP / RELP Protein (His Tag)

Mouse Regenerating Islet-Derived Protein 4 Protein

REG4 / GISP / RELP Protein Product Information

• Synonym: 2010002L15Rik, GISP, RELP
• Protein Construction: A DNA sequence encoding mouse REG4 ( NP_080604.2 ) ( Met 1 - Thr 157 ) was expressed with the polyhistide tag at the C-terminus.
• Source: Mouse
• Expression Host: Human Cells

REG4 / GISP / RELP Protein QC Testing

• Purity: > 95 % as determined by SDS-PAGE
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion and proliferation of HCT-116 human colorectal carcinoma cells ( ATCC: CCL-247 ) under low serum conditions.
  When 5 x 10⁴ cells/well are added to mouse REG4 coated plates ( 0.16 μg/mL and 100 μL/well ), > 70% cell will adhere after 60 minutes at 37℃
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Asp 23
• Molecular Mass: The recombinant mouse REG4 comprises 146 amino acids and has a predicted molecular mass of 17.5 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm REG4 is approximately 23-30 kDa due to glycosylation.
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: REG4 protein

REG4 / GISP / RELP Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

REG4 / GISP / RELP Protein Description

Mouse regenerating islet-derived protein 4, also known as REG-like protein, REG4, GISP and RELP, is a secreted protein which contains one C-type lectin domain. REG4 is involved in inflammatory and metaplastic responses of the gastrointestinal epithelium. REG4 is highly expressed in the gastrointestinal tract including the duodenum, jejunum, ileum, ileocecum, appendix, descending colon, pancreas and small intestine. It is weakly expressed in normal colon and stomach and strongly expressed in most colorectal tumors than in normal colon. It is also preferentialy expressed in mucinous tumors and in some cases neuro-endocrine tumors. REG4 protein overexpression is associated with an unfavorable response to preoperative chemoradiotherapy and may be used as a predictive biomarker clinically. REG4 is also a promising tumor marker to screen early-stage Pancreatic ductal adenocarcinoma (PDAC), and neutralization of REG4 by the antibody may offer novel potential tools for the treatment of PDAC.

References

1. Hartupee, JC. et al., 2001, Biochim. Biophys. Acta 1518: 287-93.  
2. Violette, S. et al., 2003, Int. J. Cancer 103: 185-93.   
3. Kaemaeraeinen, M. et al., 2003, Am. J. Pathol. 163: 11-20. 
4. Takehara, A. et al., 2006, Cancer Sci. 97 (11): 1191-7.
5. Eguchi, H. et al., 2009, Pancreas. 38 (7): 791-8.