REG4 / RELP / GISP Antibody (Antigen Affinity Purified)

Regenerating Islet-derived Protein 4 Antibody ( Antigen Affinity Purified )

REG4 / RELP / GISP Antibody Product Information

• Immunogen: Recombinant human REG4 protein ( Catalog#11186-H08H )
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, human cell-derived, recombinant human REG4 ( rh REG4 ; Catalog#11186-H08H ; NP_001152824.1 ; Met 1 - Pro 158 ). REG4 specific IgG was purified by human REG4 affinity chromatography

REG4 / RELP / GISP Antibody Usage Guide

• Specificity: Human REG4 / RELP / GISP
• Western blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human REG4 in WB. Using a DAB detection system, the detection limit for Human REG4 is approximately 4 ng/lane under non-reducing conditions and 2 ng/lane under reducing conditions.
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect human REG4. The detection limit for human REG4 is approximately 0.00245 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

REG4 / RELP / GISP Antibody Background

Regenerating islet-derived protein 4, also known as REG-like protein, REG4, GISP and RELP, is a secreted protein which contains one C-type lectin domain. REG4 is involved in inflammatory and metaplastic responses of the gastrointestinal epithelium. REG4 is highly expressed in the gastrointestinal tract including the duodenum, jejunum, ileum, ileocecum, appendix, descending colon, pancreas and small intestine. It is weakly expressed in normal colon and stomach and strongly expressed in most colorectal tumors than in normal colon. It is also preferentialy expressed in mucinous tumors and in some cases neuro-endocrine tumors. REG4 protein overexpression is associated with an unfavorable response to preoperative chemoradiotherapy and may be used as a predictive biomarker clinically. REG4 is also a promising tumor marker to screen early-stage Pancreatic ductal adenocarcinoma (PDAC), and neutralization of REG4 by the antibody may offer novel potential tools for the treatment of PDAC.

References

1. Hartupee, JC. et al., 2001, Biochim. Biophys. Acta 1518: 287-93.  
2. Violette, S. et al., 2003, Int. J. Cancer 103: 185-93.   
3. Kaemaeraeinen, M. et al., 2003, Am. J. Pathol. 163: 11-20.  
4. Takehara, A. et al., 2006, Cancer Sci. 97 (11): 1191-7.
5. Eguchi, H. et al., 2009, Pancreas. 38 (7): 791-8.