REG3G / PAP1B Protein (His Tag)

Regenerating islet-derived 3 gamma ( REG3G / PAP1B ) Protein

REG3G / PAP1B Protein Product Information

• Synonym: REG3G, MGC118998, MGC118999, MGC119001, PAP IB, PAP1B, PAPIB, REG-III, UNQ429
• Protein Construction: A DNA sequence encoding the human REG3G ( NP_001008388.1 ) ( Met 1 - Asp 175 ) was fused with a polyhistidine tag at the C-terminus
• Source: Human
• Expression Host: Human Cells

REG3G / PAP1B Protein QC Testing

• Purity: > 97 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Glu 27
• Molecular Mass: The recombinant human REG3G consists of 160 amino acids and migrates as an approximately 18kDa band in SDS-PAGE under reducing conditions as predicted
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: REG3G protein

REG3G / PAP1B Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

REG3G / PAP1B Protein Description

Regenerating gene (Reg), first isolated from a regenerating islet cDNA library, encodes a secretory protein with a growth stimulating effect on pancreatic beta cells. Reg and Reg-related genes which were expressed in various organs have been revealed to constitute a multigene family, the Reg family consisting of four subtypes (types I, II, III, IV) and are involved in cancers and neurodegenerative diseases. Regenerating islet-derived 3 gamma (REG3G), also known as pancreatitis-associated protein 1B ( PAP1B), is a member of the secreted Reg superfamily and contains one typical C-type lectin domain. REG III was expressed predominantly in pancreas, as well as in testis. REG3G might be a stress protein involved in the control of bacterial proliferation. It was indicated that REG3G specifically targets Gram-positive bacteria because it binds to their surface peptidoglycan layer, and serves as one of several antimicrobial peptides produced by paneth cells via stimulation of toll-like receptors (TLRs) by pathogen-associated molecular patterns (PAMPs).

References

1. Nata, K., et al., 2004, Gene. 340:161-70.
2. Laurine. E.et al., 2005, Biochim. Biophys. Acta. 1727: 177-87.
3. Clark, H.F. et,al., 2003, Genome. Res. 13: 2265-70.
4. Abreu, M.T. 2010, Nat. Rev. Immunol. 10: 131-44.