REG3G / PAP1B Antibody

REG3G / PAP1B Antibody

REG3G / PAP1B Antibody Product Information

• Immunogen: Recombinant human REG3G protein ( Catalog#11641-H08H )
• Antibody Type: Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform )
• Clone ID: 002
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was obtained from a rabbit immunized with purified, human cell-derived, recombinant human REG3G ( rh REG3G ; Catalog#11641-H08H ; NP_001008388.1 ; Met 1 - Asp 175 ).

REG3G / PAP1B Antibody Usage Guide

• Specificity: Human REG3G / PAP1B
• Western blot: This antibody can be used at 1 - 2 μg/mL with the appropriate secondary reagents to detect human REG3G in WB. Using a DAB detection system, the detection limit for human REG3G is approximately 0.125 ng/lane under non-reducing conditions and reducing conditions
• Direct ELISA: This antibody can be used at 0.1 - 0.2 μg/mL with the appropriate secondary reagents to detect human REG3G. The detection limit for human REG3G is approximately 0.00245 ng/well
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

REG3G / PAP1B Antibody Background

Regenerating gene (Reg), first isolated from a regenerating islet cDNA library, encodes a secretory protein with a growth stimulating effect on pancreatic beta cells. Reg and Reg-related genes which were expressed in various organs have been revealed to constitute a multigene family, the Reg family consisting of four subtypes (types I, II, III, IV) and are involved in cancers and neurodegenerative diseases. Regenerating islet-derived 3 gamma (REG3G), also known as pancreatitis-associated protein 1B ( PAP1B), is a member of the secreted Reg superfamily and contains one typical C-type lectin domain. REG III was expressed predominantly in pancreas, as well as in testis. REG3G might be a stress protein involved in the control of bacterial proliferation. It was indicated that REG3G specifically targets Gram-positive bacteria because it binds to their surface peptidoglycan layer, and serves as one of several antimicrobial peptides produced by paneth cells via stimulation of toll-like receptors (TLRs) by pathogen-associated molecular patterns (PAMPs).

References

1. Nata, K., et al., 2004, Gene. 340:161-70.
2. Laurine. E.et al., 2005, Biochim. Biophys. Acta. 1727: 177-87.
3. Clark, H.F. et,al., 2003, Genome. Res. 13: 2265-70.
4. Abreu, M.T. 2010, Nat. Rev. Immunol. 10: 131-44.