Mouse REG3D Gene cDNA Clone / ORF Clone

REG3D cDNA Clone Datasheet

REG3D cDNA Clone Product Information

• Gene Bank Ref.ID: NM_013893.2
• cDNA Size: 528
• Gene Synonym: Ingaprp; MGC130575; Reg3d
• Source: Mouse

REG3D cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus regenerating islet-derived 3 delta DNA.
• Vector: Vector pMD18-T Simple
• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

REG3D cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

REG3D cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

REG3D cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGTGTCTC ACAAGACCCT TCATAGCATG TCCTGGATGC TACTGTGTTG CCTGATGTCC
61       CTTTCTTGGG TACAAGGTGA ACAATCCCAG AAAAAACTGT CTTCTCCACG CATCAGCTGT
121      CCCCAAGAAG CCCAAGCTTA TGGCTCCTAT TGCTATTTAC TGATTCTGGA ACCACAGACC
181      TGGGCTAATG CAGAGATCCA CTGCCAGAAG CATTTCTCAG GACACCTGGC ATTTCTGCTC
241      ACTTATGGTG AAATTATCTT TGTGTCCTCT CTGGTGAAAA ACAGTTTGAC CACATTCCCA
301      TACATCTGGA TTGGACTCCA TGATCTGTCA CTTGGGAGTT TGCCCAATGA AAATGGATGG
361      AAGTGGAGCA GCTCTGACCC TCTGACCTTC TATAACTGGG AGATACCACC CTCCATGTCT
421      GCACACCACG GCTACTGTGC AGCTTTGTCT CAGGCCTCAG GTTATCAGAA GTGGAGAGAT
481      TATTATTGTG ACACAATATT TCCCTATGTC TGCAAATTCA AGGGTTAG

 

Quality Control:cDNA sequencing results is available upon request.