Mouse REG3D Protein (His Tag)

Mouse Regenerating Islet Derived III Delta Protein

REG3D / INGAPrP Protein Product Information

• Synonym: Ingaprp , MGC130575 , Reg3d
• Protein Construction: A DNA sequence encoding the mouse REG3D isoform 1 ( NP_038921.2 ) extracellular domain ( Met 1 - Gly 175 ) was fused with a polyhistidine tag at the C-terminus
• Source: Mouse
• Expression Host: Human Cells

REG3D / INGAPrP Protein QC Testing

• Purity: > 97 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Glu 27
• Molecular Mass: The secreted recombinant mouse REG3D comprises 160 amino acids and has a predicted molecular mass of 18.5 kDa. rmREG3D maigrates as an approximately 17 kDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: REG3D protein

REG3D / INGAPrP Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

REG3D / INGAPrP Protein Description

REG3D, also known as regenerating islet derived III delta, islet neogenesis-associated protein related protein and INGAPrP, is an 20 kDa member of the REG family of secreted calcium dependent C-type lectins. REG proteins are involved in various biological functions, including acting as a survival / growth factors. REG3D mRNA is expressed mainly in pancreatic islets and the digestive system. Regenerating gene (Reg), first isolated from a regenerating islet cDNA library, encodes a secretory protein with a growth stimulating effect on pancreatic beta cells. Reg and Reg-related genes which were expressed in various organs have been revealed to constitute a multigene family, the Reg family consisting of four subtypes (types I, II, III, IV) and are involved in cancers and neurodegenerative diseases.

References

1. Nata, K., et al., 2004, Gene. 340:161-70.
2. Laurine. E.et al., 2005, Biochim. Biophys. Acta. 1727: 177-87.
3. Abreu, M.T. 2010, Nat. Rev. Immunol. 10: 131-44.