Mouse REG3A Gene cDNA Clone / ORF Clone

REG3A cDNA Clone Datasheet

REG3A cDNA Clone Product Information

• Gene Bank Ref.ID: NM_011259.1
• cDNA Size: 528
• Gene Synonym: AV063448; Reg3a
• Source: Mouse

REG3A cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus regenerating islet-derived 3 alpha DNA.
• Vector: Vector pMD18-T Simple
• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

REG3A cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

REG3A cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

REG3A cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCTGCCTC ACCTGGTCCT CAACAGTATT TCCTGGATGC TGCTCTCCTG CCTGTTGTTT
61       GTATTTCAGG TACAAGGTGA AGACTTCCAG AAGGAAGTGC CCTCTCCACG TACCAGCTGC
121      CCCATGGGTT ACAAGGCTTA TCGCTCCCAC TGCTATGCCT TAGTTATGAC ACCTAAATCC
181      TGGTTTCAAG CAGATCTGGT CTGCCAGAAG AGACCCTCAG GACATCTCGT GTCTATTCTT
241      AGTGGAGGTG AGGCTTCCTT TGTGTCCTCC TTGGTGAACG GCAGAGTGGA CAACTACCAA
301      GACATCTGGA TTGGGCTCCA TGATCCAACA ATGGGTCAAC AACCCAATGG AGGTGGATGG
361      GAGTGGAGTA ACTCCGATGT GCTGAATTAT CTTAACTGGG ATGGGGATCC TTCCTCTACT
421      GTCAACCGTG GTCACTGTGG GAGTCTGACA GCAAGTTCAG GGTTTCTGAA GTGGGGAGAC
481      TATTACTGCG ATGGGACATT ACCATTTGTC TGCAAGTTCA AGCAGTAG

 

Quality Control:cDNA sequencing results is available upon request.