REG3A / HIP Protein (His Tag)

Regenerating Islet-Derived Protein 3-alpha ( REG3A / HIP ) Protein

REG3A / HIP Protein Product Information

• Synonym: REG3A , HIP, PAP, PAP-H, PAP1, PBCGF, REG-III, REG3V
• Protein Construction: A DNA sequence encoding the human REG3A ( Q06141-1 ) ( Met 1 - Asp 175 ) precursor was fused with a polyhistidine tag at the C-terminus
• Source: Human
• Expression Host: Human Cells

REG3A / HIP Protein QC Testing

• Purity: > 97 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Glu 27
• Molecular Mass: The recombinant human REG3A consists of 160 amino acids after removal of the signal peptide and has a predicted molecular mass of 18 kDa as estimated in SDS-PAGE under reducing conditions
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: REG3A protein

REG3A / HIP Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

REG3A / HIP Protein Description

Regenerating islet-derived protein 3-alpha, also known as Regenerating islet-derived protein III-alpha, REG-3-alpha, REG3A, and HIP, is secreted protein which contains one C-type lectin domain. REG3A is constitutively expressed in intestine. Low expression of REG3A is found in healthy pancreas. It is overexpressed during the acute phase of pancreatitis and in some patients with chronic pancreatitis. REG3A and REG1A proteins are both involved in liver and pancreatic regeneration and proliferation. REG3A is also a stress protein involved in the control of bacterial proliferation. REG3A is down-regulated in most primary human gastric cancer cells, and might be useful in the diagnosis of gastric cancer. Further characterization of the REG3A, should lead to a better understanding of the changes occurring at the molecular level during the development and progression of primary human gastric cancer.

References

1. Orelle B. et al., 1992, J. Clin. Invest. 90: 2284-2291.
2. Cavard, C. et al., 2006, Oncogene. 25 (4): 599-608.
3. Choi, B. et al., 2007, Exp Mol Med. 39 (6): 796-804.
4. Yasui, W. et al., 2009, Pathol Int. 59 (3): 121-36.