Human REG1B Gene cDNA Clone / ORF Clone

REG1B cDNA Clone Datasheet

REG1B cDNA Clone Product Information

• Gene Bank Ref.ID: NM_006507.3
• cDNA Size: 501
• Gene Synonym: REGH; REGL; PSPS2; REGI-BETA; REG1B
• Source: Human

REG1B cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens regenerating islet-derived 1 beta DNA.
• Vector: Vector pMD18-T Simple
• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

REG1B cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

REG1B cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

REG1B cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCTCAGA CCAACTCGTT CTTCATGCTG ATCTCCTCCC TGATGTTCCT GTCTCTGAGC
61       CAAGGCCAGG AGTCCCAGAC AGAGCTGCCT AATCCCCGAA TCAGCTGCCC AGAAGGCACC
121      AATGCCTATC GCTCCTACTG CTACTACTTT AATGAAGACC CTGAGACCTG GGTTGATGCA
181      GATCTCTATT GCCAGAACAT GAATTCAGGC AACCTGGTGT CTGTGCTCAC CCAGGCGGAG
241      GGTGCCTTCG TGGCCTCACT GATTAAGGAG AGTAGCACTG ATGACAGCAA TGTCTGGATT
301      GGCCTCCATG ACCCAAAAAA GAACCGCCGC TGGCACTGGA GTAGTGGGTC CCTGGTCTCC
361      TACAAGTCCT GGGACACTGG ATCCCCGAGC AGTGCTAATG CTGGCTACTG TGCAAGCCTG
421      ACTTCATGCT CAGGATTCAA GAAATGGAAG GATGAATCTT GTGAGAAGAA GTTCTCCTTT
481      GTTTGCAAGT TCAAAAACTA G

 

Quality Control:cDNA sequencing results is available upon request.